Аннотация:Background: High uptake of, and optimal adherence to Option B+ antiretroviral therapy (ART), increase the effectiveness in averting mother-to-child transmission of HIV.Option B+ ART uptake, early adherence, and associated factors need to be evaluated in Central Uganda.Methods: A mixed approaches study was carried out in six health facilities in Masaka, Mityana, and Luwero districts from October 2013 to March 2016.Questionnaires were administered to 507 HIV-positive pregnant females seeking antenatal care services.