Content of total bile acids in blood and bile of laboratory animalsстатья из журнала
Аннотация: In the human or animal organism, bile acids influence the carbohydrate and lipid metabolism and participate in the process of emulsification of food lipids, cholesterol, and fat-soluble vitamins in the intestinal lumen. Most frequently the change in level and composition of lipid acids is associated with pathologies of the hepatobiliary system. When researching the pharmacodynamic activity of some medications on experimental models of pathology of the hepatobiliary system of laboratory animals, one of the rating parameters is the level of common bile acids in different biological materials. For the analysis and interpretation of the level of common bile acids, it is necessary to consider the species of the animal, the composition of the feed used and the fasting regime. It is also important to consider the kind of analyzed biological material and an array of other factors which are capable of influencing the defined index and the research results. This article provides data on the level of common bile acids in peripheric blood and in the bile of intact mature laboratory animals. A low level of common bile acids in the blood serum was recorded throughout the experiment in comparison to data received from the bile. The received results variate significantly in dependence on the animal species and the material analyzed. Individual values of bile acids in the blood of all animals did not extend beyond 48,7 µmol/l, while in the bile intervals were within the limits of 8,2 to 8710,7 mmol/l, in dependence on the animal species. Minimal bile acids’ values in blood, as the minimal ones in the bile, were recorded in ferrets: 0–0,6 µmol/l и 226–8711 mmol/l accordingly, which is most probably linked to the nourishment of carnivore mammals, at the same time the highest values were recorded in rabbits [24,4 (16,7; 34,5) µmol/l], which can also be linked with features of digestion of the herbivores. Values of bile acids in the bile of rodents were much lower in comparison with other species, the guinea pigs had the lowest boundaries of values — 19±2 mmol/l. The conducted analysis had discovered a high variety of the level of common bile acids in blood and bile of different animal species and in bounds of one species, which can complicate the interpretation of data while rating the influence of a medication. As a result of this, while planning a research linked with the definition of the level of common bile acids, augmentation of animal sampling is necessary as well as the planning and the controlling of uniformity of the selection of biological material.
Год издания: 2023
Авторы: E.V. Simonova, K.T. Sultanova, Н. М. Фаустова, А. Romanenko
Источник: Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)
Ключевые слова: Drug Transport and Resistance Mechanisms, Liver Disease Diagnosis and Treatment, Drug-Induced Hepatotoxicity and Protection
Открытый доступ: bronze
Том: 6
Выпуск: 4