Abstracts ADDRM 2023статья из журнала
Аннотация: BACKGROUNDLow magnesium levels, which commonly occurs in people with type 2 diabetes, are associated with pro-inflammatory response.In this study, we investigated whether magnesium supplementation in people with type 2 diabetes and low magnesium levels would benefit their immune system. METHODSIn this double-blind, placebo-controlled, two-period crossover study, 12 people with type 2 diabetes and low serum magnesium levels were recruited to assess the effect of magnesium supplementation (15 mmol/day) on immune function.Blood was drawn at the end of each treatment period to measure the levels of circulating inflammatory proteins, circulating immune cell numbers, and ex vivo immune cell functionality.Circulating inflammatory protein levels were measured using Olink inflammation panel.Flow cytometry was used to determine the whole blood immune cell composition, as well as ex vivo functionality of peripheral blood mononuclear cells. RESULTSMagnesium supplementation increased the levels of circulating magnesium as compared to placebo (0.69 ± 0.02 and 0.76 ± 0.02, respectively).We found no change in circulating inflammatory protein levels post-magnesium supplementation.The number of circulating immune cells were also not affected by magnesium supplementation.However, we observed lower ex vivo production of IFNy and IL4/ IL5/13 from CD4+ T-cells (p = 0.011 & p = 0.015), and IFNy production from CD8+ T-cells (p = 0.008) aer PMA+ionomycin supplementation.The effect of magnesium supplementation exclusively affected lymphocytes, as we observed no change in ex vivo monocyte functionality post-supplementation. CONCLUSIONMagnesium supplementation attenuates pro-inflammatory response in CD4+ and CD8+ T-cells in people with type 2 diabetes and low serum magnesium levels.
Год издания: 2023
Издательство: Bohn Stafleu van Loghum
Источник: Nederlands Tijdschrift voor Diabetologie
Открытый доступ: hybrid
Том: 21
Выпуск: 4
Страницы: 8–60