Proteome Remodeling of the Eye Lens at 50 Years Identified With Data-Independent Acquisitionстатья из журнала
Аннотация: The eye lens is responsible for focusing and transmitting light to the retina. The lens does this in the absence of organelles, yet maintains transparency for at least 5 decades before onset of age-related nuclear cataract (ARNC). It is hypothesized that oxidative stress contributes significantly to ARNC formation. It is in addition hypothesized that transparency is maintained by a microcirculation system that delivers antioxidants to the lens nucleus and exports small molecule waste. Common data-dependent acquisition methods are hindered by dynamic range of lens protein expression and provide limited context to age-related changes in the lens. In this study, we utilized data-independent acquisition mass spectrometry to analyze the urea-insoluble membrane protein fractions of 16 human lenses subdivided into three spatially distinct lens regions to characterize age-related changes, particularly concerning the lens microcirculation system and oxidative stress response. In this pilot cohort, we measured 4788 distinct protein groups, 46,681 peptides, and 7592 deamidated sequences, more than in any previous human lens data-dependent acquisition approach. Principally, we demonstrate that a significant proteome remodeling event occurs at approximately 50 years of age, resulting in metabolic preference for anaerobic glycolysis established with organelle degradation, decreased abundance of protein networks involved in calcium-dependent cell-cell contacts while retaining networks related to oxidative stress response. Furthermore, we identified multiple antioxidant transporter proteins not previously detected in the human lens and describe their spatiotemporal and age-related abundance changes. Finally, we demonstrate that aquaporin-5, among other proteins, is modified with age by post-translational modifications including deamidation and truncation. We suggest that the continued accumulation of each of these age-related outcomes in proteome remodeling contribute to decreased fiber cell permeability and result in ARNC formation.
Год издания: 2022
Авторы: Lee S. Cantrell, Romell B. Gletten, Kevin L. Schey
Издательство: Elsevier BV
Источник: Molecular & Cellular Proteomics
Ключевые слова: Connexins and lens biology, Advanced Glycation End Products research, S100 Proteins and Annexins
Другие ссылки: Molecular & Cellular Proteomics (PDF)
Molecular & Cellular Proteomics (HTML)
PubMed Central (HTML)
bioRxiv (Cold Spring Harbor Laboratory) (HTML)
PubMed (HTML)
Molecular & Cellular Proteomics (HTML)
PubMed Central (HTML)
bioRxiv (Cold Spring Harbor Laboratory) (HTML)
PubMed (HTML)
Открытый доступ: gold
Том: 22
Выпуск: 1
Страницы: 100453–100453