Аннотация:Background and objective: Alveolar bone resorption due to chronic inflammation is the main symptom of periodontitis.Conventional mouse periodontitis models require improvement as a model of chronic inflammation, particularly with respect to the method of inducing inflammation during the observation period.In this study, alveolar bone resorption was induced by a method which caused little mechanical irritation, and periodontitis progression in young and old mice was compared.Materials and Methods: Lipopolysaccharide(LPS)from Porphyromonas gingivalis was administered 12 times(twice per week)on the palatal gingiva of 8-or 24-weeks old C57BL/6J mice.The LPS was injected by a microsyringe(33 G) , and the dose was 20 μg per 1 shot.Maxilla samples were collected 1 week and 4 weeks after the final administration, and micro-CT scans and tissue slices were prepared.Results: Injection of LPS by the microsyringe did not require strong external force.Results from the micro-CT showed that bone resorption stimulated by LPS was greater in the older mice than in the young mice.In the comparison between 1 week and 4 weeks after the final administration, the aged mice demonstrated more bone resorption later than the young mice in after 4 weeks.Conclusion: LPS administration with a micro-syringe caused alveolar bone resorption in both young and old mice.In older mice, bone resorption was observed continuously in 4 weeks after LPS administration.
Год издания:2020
Источник:Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)
