Аннотация:Background: In asthma, CD4 + T cells are selectively recruited into the bronchial mucosa.CD4 + T cells consist of different subsets that express lineage-specific transcription factors and play different roles either in initiating and supporting the development of immune response, but also in orchestrating and regulating them.oBjectives: The aim of our study was to evaluate the effect of T cellsbronchial fibroblasts interaction on CD4 + T cell phenotype.Methods: Human bronchial fibroblasts were isolated from mild steroid naïve asthmatics and nonatopic healthy controls.CD4 + T cells were purified from the peripheral blood of healthy and asthmatic subjects.Co-culture of confluent healthy (HF) or asthmatic bronchial fibroblasts (AF) with T cells were performed.CD4 + T cell total RNA was purified and GATA-3, Foxp3 and RORc expression was detected by quantitative PCR.Th17 (IL-17,IL-22) lineage-specific cytokines profile were also evaluated.results: Co-culture of T cells with bronchial fibroblasts significantly stimulated RORc in asthmatic T cells only, whereas Foxp3 and GATA-3 were not affected in both asthmatic and healthy T cells.IL-6 and IL-23 expression either by AF and HF were also significantly increased by the coculture when, TGF-β expression was not affected.In CD4 + T cells, IL-17 and IL-22, Th17 lineage-specific cytokines were significantly increased by the coculture with AF. conclusion: Interaction between bronchial fibroblasts and T cells seems to specifically promote Th17 cells profile in asthma.These results suggest that cellular interactions particularly between T cells and fibroblasts may play a pivotal role in the regulation of the inflammatory response in asthma.
Год издания:2010
Издательство:Pulsus Group
Источник:Canadian Respiratory Journal
Ключевые слова:IL-33, ST2, and ILC Pathways, Asthma and respiratory diseases
