Аннотация:Brucellosis is zoonotic disease, more contagious and chronic disease with absence of specific clinical features which is difficult to diagnose clinically. Important test routinely used for diagnosing this disease is serological test although isolation of organism and Polymerase chain reaction used for definitive diagnosis. The present study was done to know the seroprevalence of human brucellosis in and around Chitradurga. Out of 154 Blood samples referred by clinicians for serodiagnosis of brucellosis to Microbiology laboratory 69 samples were included for evaluation of Brucella. Demographic, clinical and other required data of patients extracted from medical case records. Serum was separated from samples and Rose Bengal plate test (RBPT) carried out by both qualitative and semiquantitative method. The overall seroprevalence of brucellosis in the study was 11.59%. Disease was more commonly seen among 23-39 years age group. Slightly higher cases identifies among males than females. Fever with no apparent clinical features was present in 8/13(61.5%) patients. 69.2% cases had history of animal contact and living in rural areas. RBPT semiquantitative test Titres of 1:2 were detected in majority (38.4%) of samples. Conclusion: Though culture is gold standard, serology remains simple, cost effective, best diagnostic method for Brucellosis. Clinicians should have high degree of suspicion of brucellosis with history of animal contact, or living in rural areas. Use of the rose Bengal test as the sole technique for the diagnosis of brucellosis can be considered.
