Аннотация:By using indirect immunofluorescence and confocal microscopy, we documented changes in the distribution of elongation factor-lu (EF-la), actin, and microtubules during the development of maize endosperm cells. In older interphase cells actively forming statch grains and protein bodies, the protein bodies are enmeshed in EF-la and actin and are found juxtaposed with a multidirectional array of microtubules. Actin and EF-la appear to exist in a complex, because we observed that the two are colocalized, and treatment with cytochalasln D resulted in the redistribution of EF-1a. These dhta suggest that EF-la and actin are associated in maize endosperm cells and may help to explain the basis of the correlation we found between the concentration of EM a and lysine content. The data also support the hypothesis that the cytoskeleton plays a role in.storage protein deposition. The distributions of EF-la, actin, and microtubules change during development. We observed that in young cells before the accumulation of statch and storage protein, EF-la, actin, and microtubules are found mainly in the cell cortex or in association with nuclei.
