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The Cocaine andOxycodone Biobanks,Two Repositories fromGenetically Diverseand BehaviorallyCharacterized Ratsfor the Study ofAddiction Lieselot L. G. CarretteGiordano de GuglielmoMarsida Kallupi2021 год

The Cocaine and Oxycodone Biobanks, Two Repositories from Genetically Diverse and Behaviorally Characterized Rats for the Study of Addiction
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Аннотация: The rat oxycodone and cocaine biobanks contain samples that vary by genotypes (by using genetically diverse genotyped HS rats), phenotypes (by measuring addiction-like behaviors in an advanced SA model), timepoints (samples are collected longitudinally before, during, and after SA, and terminally at three different timepoints in the addiction cycle: intoxication, withdrawal, and abstinence or without exposure to drugs through age-matched naive rats), samples collected (organs, cells, biofluids, feces), preservation (paraformaldehyde-fixed, snap-frozen, or cryopreserved) and application (proteomics, transcriptomics, microbiomics, metabolomics, epigenetics, anatomy, circuitry analysis, biomarker discovery, etc. Substance use disorders (SUDs) are pervasive in our society and have substantial personal and socioeconomical costs. A critical hurdle in identifying biomarkers and novel targets for medication development is the lack of resources for obtaining biological samples with a detailed behavioral characterization of SUD. Moreover, it is nearly impossible to find longitudinal samples. As part of two ongoing large-scale behavioral genetic studies in heterogeneous stock (HS) rats, we have created two preclinical biobanks using well-validated long access (LgA) models of intravenous cocaine and oxycodone self-administration (SA) and comprehensive characterization of addiction-related behaviors. The genetic diversity in HS rats mimics diversity in the human population and includes individuals that are vulnerable or resilient to compulsive-like responding for cocaine or oxycodone. Longitudinal samples are collected throughout the experiment, before exposure to the drug, during intoxication, acute withdrawal, and protracted abstinence, and include naive, age-matched controls. Samples include, but are not limited to, blood plasma, feces and urine, whole brains, brain slices and punches, kidney, liver, spleen, ovary, testis, and adrenal glands. Three preservation methods (fixed in formaldehyde, snap-frozen, or cryopreserved) are used to facilitate diverse downstream applications such as proteomics, metabolomics, transcriptomics, epigenomics, microbiomics, neuroanatomy, biomarker discovery, and other cellular and molecular approaches. To date, >20,000 samples have been collected from over 1000 unique animals and made available free of charge to non-profit institutions through https://www.cocainebiobank.org/ and https://www.oxycodonebiobank.org/ .
Год издания: 2021
Авторы: Lieselot L. G. Carrette, Giordano de Guglielmo, Marsida Kallupi, Lisa Maturin, Molly Brennan, Brent Boomhower, Dana Conlisk, Sharona Sedighim, Lani Tieu, McKenzie J. Fannon, Nathan Velarde, Angelica Martinez, Jenni Kononoff, Adam Kimbrough, Sierra Simpson, Lauren C. Smith, Kokila Shankar, Francisco J. Ramírez, Apurva S. Chitre, Bonnie Lin, Oksana Polesskaya, Leah C. Solberg Woods, Abraham A. Palmer, Olivier George
Издательство: Society for Neuroscience
Источник: eNeuro
Ключевые слова: Neurotransmitter Receptor Influence on Behavior, Receptor Mechanisms and Signaling, Memory and Neural Mechanisms
Другие ссылки: eNeuro (PDF)
eNeuro (HTML)
eScholarship (California Digital Library) (PDF)
eScholarship (California Digital Library) (HTML)
PubMed Central (HTML)
PubMed (HTML)