Initial evaluation of a mobile SARS-CoV-2 RT-LAMP testing strategypreprint
Аннотация: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) control in the United States remains hampered, in part, by testing limitations. We evaluated a simple, outdoor, mobile, colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay workflow where self-collected saliva is tested for SARS-CoV-2 RNA. From July 16 to November 19, 2020, 4,704 surveillance samples were collected from volunteers and tested for SARS-CoV-2 at 5 sites. A total of 21 samples tested positive for SARS-CoV-2 by RT-LAMP; 12 were confirmed positive by subsequent quantitative reverse-transcription polymerase chain reaction (qRT-PCR) testing, while 8 were negative for SARS-CoV-2 RNA, and 1 could not be confirmed because the donor did not consent to further molecular testing. We estimated the RT-LAMP assay's false-negative rate from July 16 to September 17, 2020 by pooling residual heat-inactivated saliva that was unambiguously negative by RT-LAMP into groups of 6 or less and testing for SARS-CoV-2 RNA by qRT-PCR. We observed a 98.8% concordance between the RT-LAMP and qRT-PCR assays, with only 5 of 421 RT-LAMP negative pools (2,493 samples) testing positive in the more sensitive qRT-PCR assay. Overall, we demonstrate a rapid testing method that can be implemented outside the traditional laboratory setting by individuals with basic molecular biology skills and can effectively identify asymptomatic individuals who would not typically meet the criteria for symptom-based testing modalities.
Год издания: 2020
Авторы: Christina M. Newman, Mitchell D. Ramuta, Matthew T. McLaughlin, Roger W. Wiseman, Julie A. Karl, Dawn M. Dudley, Miranda R. Stauss, Robert James Maddox, Andrea M. Weiler, Mason I. Bliss, Katrina N. Fauser, Luis A. Haddock, Cecilia G. Shortreed, Amelia K. Haj, Molly A. Accola, Anna S. Heffron, Hailey E. Bussan, Matthew R. Reynolds, Olivia E. Harwood, Ryan V. Moriarty, Laurel M. Stewart, Chelsea M. Crooks, Trent M. Prall, Emma K. Neumann, Elizabeth D. Somsen, Corrie B. Burmeister, Kristi L. Hall, William M. Rehrauer, Thomas C. Friedrich, Shelby L. O’Connor, David H. O’Connor
Издательство: Cold Spring Harbor Laboratory
Источник: medRxiv (Cold Spring Harbor Laboratory)
Ключевые слова: SARS-CoV-2 detection and testing, Biosensors and Analytical Detection, Advanced biosensing and bioanalysis techniques
Другие ссылки: medRxiv (Cold Spring Harbor Laboratory) (HTML)
www.ncbi.nlm.nih.gov (PDF)
doi.org (HTML)
PubMed Central (HTML)
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www.ncbi.nlm.nih.gov (PDF)
doi.org (HTML)
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