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Mesenchymal StromalCell Bioreactor forEx Vivo Reprogrammingof Human Immune Cells Christine AllenNatalie VaninovMatthew Li2020 год

Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells
статья из журнала

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: Abstract Bone marrow mesenchymal stromal cells (MSCs) have been studied for decades as potent immunomodulators. Clinically, they have shown some promise but with limited success. Here, we report the ability of a scalable hollow fiber bioreactor to effectively maintain ideal MSC function as a single population while also being able to impart an immunoregulatory effect when cultured in tandem with an inflamed lymphocyte population. MSCs were seeded on the extraluminal side of hollow fibers within a bioreactor where they indirectly interact with immune cells flowing within the lumen of the fibers. MSCs showed a stable and predictable metabolite and secreted factor profile during several days of perfusion culture. Exposure of bioreactor-seeded MSCs to inflammatory stimuli reproducibly switched MSC secreted factor profiles and altered microvesicle composition. Furthermore, circulating, activated human peripheral blood mononuclear cells (PBMCs) were suppressed by MSC bioreactor culture confirmed by a durable change in their immunophenotype and function. This platform was useful to study a model of immobilized MSCs and circulating immune cells and showed that monocytes play an important role in MSC driven immunomodulation. This coculture technology can have broad implications for use in studying MSC-immune interactions under flow conditions as well as in the generation of ex vivo derived immune cellular therapeutics.
Год издания: 2020
Авторы: Christine Allen, Natalie Vaninov, Matthew Li, Sunny Nguyen, Maneet Singh, Peter Igo, Arno W. Tilles, Brian O’Rourke, Brian L. K. Miller, Biju Parekkadan, Rita N. Bárcia
Издательство: Nature Portfolio
Источник: Scientific Reports
Ключевые слова: Mesenchymal stem cell research, Extracellular vesicles in disease, Tissue Engineering and Regenerative Medicine
Другие ссылки: Scientific Reports (PDF)
Scientific Reports (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)