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SARS‐CoV‐2Seroconversion inHumans: A DetailedProtocol for aSerological Assay,Antigen Production,and Test Setup Daniel StadlbauerFatima AmanatVeronika Chromikova2020 год

SARS‐CoV‐2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup
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Аннотация: In late 2019, cases of atypical pneumonia were detected in China. The etiological agent was quickly identified as a betacoronavirus (named SARS-CoV-2), which has since caused a pandemic. Several methods allowing for the specific detection of viral nucleic acids have been established, but these only allow detection of the virus during a short period of time, generally during acute infection. Serological assays are urgently needed to conduct serosurveys, to understand the antibody responses mounted in response to the virus, and to identify individuals who are potentially immune to re-infection. Here we describe a detailed protocol for expression of antigens derived from the spike protein of SARS-CoV-2 that can serve as a substrate for immunological assays, as well as a two-stage serological enzyme-linked immunosorbent assay (ELISA). These assays can be used for research studies and for testing in clinical laboratories. © 2020 The Authors. Basic Protocol 1: Mammalian cell transfection and protein purification Basic Protocol 2: A two-stage ELISA for high-throughput screening of human serum samples for antibodies binding to the spike protein of SARS-CoV-2.
Год издания: 2020
Авторы: Daniel Stadlbauer, Fatima Amanat, Veronika Chromikova, Kaijun Jiang, Shirin Strohmeier, Guha Asthagiri Arunkumar, Jessica Tan, Disha Bhavsar, Christina Capuano, Ericka Kirkpatrick, Philip Meade, Ruhi Nichalle Brito, Su Hui Catherine Teo, Meagan McMahon, Viviana Simon, Florian Krammer
Издательство: Wiley
Источник: Current Protocols in Microbiology
Ключевые слова: SARS-CoV-2 and COVID-19 Research, SARS-CoV-2 detection and testing, COVID-19 Clinical Research Studies
Другие ссылки: Current Protocols in Microbiology (PDF)
Current Protocols in Microbiology (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)