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Serum IgGAnti-Toxoplasmagondii AntibodyConcentrations Do NotCorrelate Nested PCRResults in BloodDonors Fabiana NakashimaValquíria Sousa PardoMarcos Paulo Miola2020 год

Serum IgG Anti-Toxoplasma gondii Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors
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Аннотация: Background: Toxoplasma gondii infects millions of individuals worldwide. This protozoan is food and water-borne transmitted but blood transfusion and organ transplantation constitute alternative forms for transmission. However, the influence of IgG anti-T. gondii antibodies in molecular analysis carried out in peripheral blood still remain unclear. This study aimed to investigate the serum IgG anti-Toxoplasma gondii antibody concentrations correlate Nested PCR results in blood donors. Methods: 750 blood donors were enrolled. IgM and IgG anti-T. gondii antibodies were assessed by ELISA (DiaSorin, Italy). Nested PCR was performed with primers JW62/JW63 (288 bp) and B22/B23 (115bp) of the T. gondii B1 gene. The mean values of IgG concentration were compared for PCR positive and PCR Negative blood donors using the t test or Mann-Withney according to the normal distribution (p-value ≤ 0.05). Results: 361 (48.1%) blood donors presented positive serology as follow: IgM+/IgG-: 5 (0.6%); IgM+/IgG+: 21 (2.8%); IgM-IgG+: 335 (44.7%);] and 389 (51.9%), negative serology. From 353 blood donors with positive serology tested, the Nested PCR was positive in 38 (10.8%) and negative in 315 (89.2%). There were no differences statistically significant between the mean values of serum IgG anti-T. gondii antibody concentrations and the Nested PCR results. Conclusions. In conclusion, our data show that variations in the serum IgG anti-T. gondii antibody concentrations do not correlate T. gondii parasitemia detected by Nested PCR in chronically infected healthy blood donors.
Год издания: 2020
Авторы: Fabiana Nakashima, Valquíria Sousa Pardo, Marcos Paulo Miola, F. Murata, Natália Juliana Paduan, S. Longo, Cinara Cássia Brandão de Mattos, Vera Lúcia Pereira‐Chioccola, Octávio Ricci, Luíz Carlos de Mattos
Издательство: Frontiers Media
Источник: Frontiers in Cellular and Infection Microbiology
Ключевые слова: Toxoplasma gondii Research Studies, Cytomegalovirus and herpesvirus research, Parvovirus B19 Infection Studies
Другие ссылки: Frontiers in Cellular and Infection Microbiology (PDF)
Frontiers in Cellular and Infection Microbiology (HTML)
DOAJ (DOAJ: Directory of Open Access Journals) (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
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