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Metabolic Profilingof Saponin-RichOphiopogon japonicusRoots Based on 1H NMRand HPTLC Platforms Yanhui GeXiaojia ChenDejan Gođevac2019 год

Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms
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Аннотация: Ideally, metabolomics should deal with all the metabolites that are found within cells and biological systems. The most common technologies for metabolomics include mass spectrometry, and in most cases, hyphenated to chromatographic separations (liquid chromatography- or gas chromatography-mass spectrometry) and nuclear magnetic resonance spectroscopy. However, limitations such as low sensitivity and highly congested spectra in nuclear magnetic resonance spectroscopy and relatively low signal reproducibility in mass spectrometry impede the progression of these techniques from being universal metabolomics tools. These disadvantages are more notorious in studies of certain plant secondary metabolites, such as saponins, which are difficult to analyse, but have a great biological importance in organisms. In this study, high-performance thin-layer chromatography was used as a supplementary tool for metabolomics. A method consisting of coupling 1H nuclear magnetic resonance spectroscopy and high-performance thin-layer chromatography was applied to distinguish between Ophiopogon japonicus roots that were collected from two growth locations and were of different ages. The results allowed the root samples from the two growth locations to be clearly distinguished. The difficulties encountered in the identification of the marker compounds by 1H nuclear magnetic resonance spectroscopy was overcome using high-performance thin-layer chromatography to separate and isolate the compounds. The saponins, ophiojaponin C or ophiopogonin D, were found to be marker metabolites in the root samples and proved to be greatly influenced by plant growth location, but barely by age variation. The procedure used in this study is fully described with the purpose of making a valuable contribution to the quality control of saponin-rich herbal drugs using high-performance thin-layer chromatography as a supplementary analytical tool for metabolomics research.
Год издания: 2019
Авторы: Yanhui Ge, Xiaojia Chen, Dejan Gođevac, Paula Carolina Pires Bueno, Luis Francisco Salomé-Abarca, Young Pyo Jang, Mei Wang, Young Hae Choi
Издательство: Thieme Medical Publishers (Germany)
Источник: Planta Medica
Ключевые слова: Ginseng Biological Effects and Applications, Traditional Chinese Medicine Analysis, Natural product bioactivities and synthesis
Другие ссылки: Planta Medica (HTML)
Leiden Repository (Leiden University) (PDF)
Leiden Repository (Leiden University) (HTML)
PubMed (HTML)