1. Главная
  2. Ресурсы
  3. Библиотечный поиск
18S rRNAmetabarcoding dietanalysis of apredatory fishcommunity acrossseasonal changes inprey availability Justin M. WaraniakTerence L. MarshKim T. Scribner2019 год

18S rRNA metabarcoding diet analysis of a predatory fish community across seasonal changes in prey availability
статья из журнала

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: Predator-prey relationships are important ecological interactions, affecting biotic community composition and energy flow through a system, and are of interest to ecologists and managers. Morphological diet analysis has been the primary method used to quantify the diets of predators, but emerging molecular techniques using genetic data can provide more accurate estimates of relative diet composition. This study used sequences from the 18S V9 rRNA barcoding region to identify prey items in the gastrointestinal (GI) tracts of predatory fishes. Predator GI samples were taken from the Black River, Cheboygan Co., MI, USA (n = 367 samples, 12 predator species) during periods of high prey availability, including the larval stage of regionally threatened lake sturgeon (Acipenser fulvescens Rafinesque 1817) in late May/early June of 2015 and of relatively lower prey availability in early July of 2015. DNA was extracted and sequenced from 355 samples (96.7%), and prey DNA was identified in 286 of the 355 samples (80.6%). Prey were grouped into 33 ecologically significant taxonomic groups based on the lowest taxonomic level sequences that could be identified using sequences available on GenBank. Changes in the makeup of diet composition, dietary overlap, and predator preference were analyzed comparing the periods of high and low prey abundance. Some predator species exhibited significant seasonal changes in diet composition. Dietary overlap was slightly but significantly higher during the period of high prey abundance; however, there was little change in predator preference. This suggests that change in prey availability was the driving factor in changing predator diet composition and dietary overlap. This study demonstrates the utility of molecular diet analysis and how temporal variability in community composition adds complexity to predator-prey interactions.
Год издания: 2019
Авторы: Justin M. Waraniak, Terence L. Marsh, Kim T. Scribner
Издательство: Wiley
Источник: Ecology and Evolution
Ключевые слова: Environmental DNA in Biodiversity Studies, Identification and Quantification in Food, Fish Ecology and Management Studies
Другие ссылки: Ecology and Evolution (PDF)
Ecology and Evolution (HTML)
DOAJ (DOAJ: Directory of Open Access Journals) (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)