Аннотация:N‐glycosylation is one of the predominant modifications of eukaryotic proteins. It is catalyzed by oligosaccharyl transferase ( OST ), an eight‐subunit protein complex in the endoplasmic reticulum membrane. OST transfers the oligosaccharide from a lipid‐linked donor ( LLO ) to the Asn‐Xaa‐Ser/Thr sequon of nascent polypeptide, usually cotranslationally by partnering with the ribosome and the translocon. We and two other groups have recently determined high‐resolution cryo‐ EM structures of the yeast and mammalian OST complexes. In this Structural Snapshot, we describe the molecular mechanism of eukaryotic OST and its interaction with the translocon.
