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The substratespecificity ofeukaryotic cytosolicchaperonin CCT Keith R. Willison2018 год

The substrate specificity of eukaryotic cytosolic chaperonin CCT
review

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Аннотация: The cytosolic chaperonin CCT (chaperonin containing TCP-1) is an ATP-dependent double-ring protein machine mediating the folding of members of the eukaryotic cytoskeletal protein families. The actins and tubulins are obligate substrates of CCT because they are completely dependent on CCT activity to reach their native states. Genetic and proteomic analysis of the CCT interactome in the yeast Saccharomyces cerevisiae revealed a CCT network of approximately 300 genes and proteins involved in many fundamental biological processes. We classified network members into sets such as substrates, CCT cofactors and CCT-mediated assembly processes. Many members of the 7-bladed propeller family of proteins are commonly found tightly bound to CCT isolated from human and plant cells and yeasts. The anaphase promoting complex (APC/C) cofactor propellers, Cdh1p and Cdc20p, are also obligate substrates since they both require CCT for folding and functional activation. In vitro translation analysis in prokaryotic and eukaryotic cell extracts of a set of yeast propellers demonstrates their highly differential interactions with CCT and GroEL (another chaperonin). Individual propeller proteins have idiosyncratic interaction modes with CCT because they emerged independently with neo-functions many times throughout eukaryotic evolution. We present a toy model in which cytoskeletal protein biogenesis and folding flux through CCT couples cell growth and size control to time dependent cell cycle mechanisms. This article is part of a discussion meeting issue ‘Allostery and molecular machines’.
Год издания: 2018
Авторы: Keith R. Willison
Издательство: Royal Society
Источник: Philosophical Transactions of the Royal Society B Biological Sciences
Ключевые слова: Heat shock proteins research, Protein Structure and Dynamics, Enzyme Structure and Function
Другие ссылки: Philosophical Transactions of the Royal Society B Biological Sciences (PDF)
Philosophical Transactions of the Royal Society B Biological Sciences (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
Spiral (Imperial College London) (PDF)
Spiral (Imperial College London) (HTML)
PubMed (HTML)