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Group 2 InnateLymphoid CellsExhibit a DynamicPhenotype in AllergicAirway Inflammation Bobby W. S. LiRalph StadhoudersMarjolein J. W. de Bruijn2017 год

Group 2 Innate Lymphoid Cells Exhibit a Dynamic Phenotype in Allergic Airway Inflammation
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Аннотация: Group 2 innate lymphoid cells (ILC2) are implicated in allergic asthma as an early innate source of the type 2 cytokines IL-5 and IL-13. However, their induction in house dust mite (HDM)-mediated airway inflammation additionally requires T cell activation. It is currently unknown whether phenotypic differences exist between ILC2s that are activated in a T cell-dependent or T cell-independent fashion. Here, we compared ILC2s in IL-33- and HDM-driven airway inflammation. Using flow cytometry, we found that surface expression levels of various markers frequently used to identify ILC2s were dependent on their mode of activation, highly variable over time, and differed between tissue compartments, including bronchoalveolar lavage (BAL) fluid, lung, draining lymph nodes, and spleen. Whereas in vivo IL-33-activated BAL fluid ILC2s exhibited an almost uniform CD25+CD127+T1/ST2+ICOS+KLRG1+ phenotype, at a comparable time point after HDM exposure BAL fluid ILC2s had a very heterogeneous surface marker phenotype. A major fraction of HDM-activated ILC2s were CD25lowCD127+T1/ST2low ICOSlowKLRG1low, but nevertheless had the capacity to produce large amounts of type 2 cytokines. HDM-activated CD25low ILC2s in BAL fluid and lung rapidly reverted to CD25high ILC2s upon in vivo stimulation with IL-33. Genome-wide transcriptional profiling of BAL ILC2s revealed ~1,600 differentially expressed genes: HDM-stimulated ILC2s specifically expressed genes involved in the regulation of adaptive immunity through B and T cell interactions, whereas IL-33-stimulated ILC2s expressed high levels of proliferation-related and cytokine genes. In both airway inflammation models ILC2s were present in the lung submucosa close to epithelial cells, as identified by confocal microscopy. In chronic HDM-driven airway inflammation ILC2s were also found inside organized cellular infiltrates near T cells. Collectively, our findings show that ILC2s are phenotypically more heterogeneous than previously thought, whereby their surface marker and gene expression profile are highly dynamic.
Год издания: 2017
Авторы: Bobby W. S. Li, Ralph Stadhouders, Marjolein J. W. de Bruijn, Melanie Lukkes, Dior M. J. M. Beerens, Maarten D. Brem, Alex KleinJan, Ingrid M. Bergen, Heleen Vroman, Mirjam Kool, Wilfred F. J. van IJcken, Tata Nageswara Rao, Hans Jörg Fehling, Rudi W. Hendriks
Издательство: Frontiers Media
Источник: Frontiers in Immunology
Ключевые слова: IL-33, ST2, and ILC Pathways, Immune Cell Function and Interaction, Eosinophilic Esophagitis
Другие ссылки: Frontiers in Immunology (PDF)
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DOAJ (DOAJ: Directory of Open Access Journals) (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
Data Archiving and Networked Services (DANS) (PDF)
Data Archiving and Networked Services (DANS) (HTML)
EUR Research Repository (Erasmus University Rotterdam) (PDF)
EUR Research Repository (Erasmus University Rotterdam) (HTML)
EUR Research Repository (Erasmus University Rotterdam) (HTML)
PubMed Central (HTML)
PubMed (HTML)