1. Главная
  2. Ресурсы
  3. Библиотечный поиск
The influence ofcaffeinesupplementation andconcerted utilizationof enzymatic andmechanical semenliquefaction onfreezability ofdromedary camel… Khalid Ahmed El-Bahrawy2017 год

The influence of caffeine supplementation and concerted utilization of enzymatic and mechanical semen liquefaction on freezability of dromedary camel spermatozoa
статья из журнала

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: Assisted reproductive technologies have been reported to improve reproductive efficiency and genetic potential in camelids. Two experiments were carried out to determine efficiency of centrifugation in the presence of a mucolytic agent for liquefaction of dromedary semen. In the first experiment, three groups, namely: I. Tris lactose (TL, control), II. Tris lactose supplemented with amylase (TL_A) and III. Tris lactose supplemented with amylase followed by seminal plasma removal via centrifugation (TL_A_Cent.) and re-suspension into enzyme free TL media. After equilibration, control group recorded 62.85 ± 4.28% motility and 1.3 ± 0.13 viscosity score, while TL_A group values were 72.88 ± 3.30% and 0.83 ± 0.07%, respectively. TL_A_Cent. group showed significant viscosity reduction (0.33 ± 0.05) and motility decline 47.85 ± 3.04% with increment in abnormalities and detached acrosome. The second experiment investigated the effect of caffeine addition to tolerate enzymatic and mechanical stress. Using 4 mM caffeine in amylase-treated semen (TL_AC) improved post-thaw motility 50.0 ± 1.29% and recovery rate 77.8 ± 3.83% compared to the control (40.17 ± 2.79% and 62.55 ± 8.39%), respectively. Caffeine supplemented centrifuged samples (TL_AC_Cent.) showed superiority (P < .05) in post-thaw motility and recovery rate (38.33 ± 6.41%, 62.76 ± 8.10%) compared to centrifuged samples TL_A_Cent. without caffeine addition (25.00 ± 2.88% and 40.47 ± 3.48%), respectively. Sperm kinetics showed that TL_A exhibited high (p < .05) values for mostly all sperm kinetics. Caffeine treatments showed superiority in velocity curved line (VCL, µm/s) 94.24 ± 8.44 for TL_AC and 104.25 ± 8.72 for the TL_AC_Cent. group compared to 86.8 ± 5.54 for TL_A, and 85.73 ± 5.99 for the TL_A_Cent. groups. In conclusion, preforming a combined enzymatic-mechanical protocol in the presence of an antioxidant may be crucial for refinement of camel semen cryopreservation.
Год издания: 2017
Авторы: Khalid Ahmed El-Bahrawy
Издательство: Taylor & Francis
Источник: International Journal of Veterinary Science and Medicine
Ключевые слова: Sperm and Testicular Function, Reproductive Biology and Fertility, Bee Products Chemical Analysis
Другие ссылки: International Journal of Veterinary Science and Medicine (PDF)
International Journal of Veterinary Science and Medicine (HTML)
DOAJ (DOAJ: Directory of Open Access Journals) (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)