Аннотация:This chapter focuses on the development of the structures that form an ectomycorrhiza, which is a complex organ formed not only of two individuals, but also of two fungal pseudo-tissues that are easily distinguishable under a microscope. The development of ectomycorrhizae involves a series of events that have been elucidated through the use of in vitro mycorrhizal systems that allow the different stages involved in this process to be separated. In the vicinity of a mycorrhizal fungus, the root architecture of the host plant may be profoundly modified by stimulation of lateral short root development. Auxin accumulation at the root apex and redistribution, in response to fungal signaling molecules during the early stage of interaction, may stimulate the process. The chapter considers the aspects leading to the establishment of a functioning ectomycorrhiza. It points out the highly dynamic development of individual ectomycorrhizae, which results in a kind of metabolic zonation.