1. Главная
  2. Ресурсы
  3. Библиотечный поиск
Human dental folliclecells expressembryonic,mesenchymal andneural stem cellsmarkers Rodrigo Lopes de LimaRosenilde C. Holanda-AfonsoVivaldo Moura‐Neto2016 год

Human dental follicle cells express embryonic, mesenchymal and neural stem cells markers
статья из журнала

Страница публикацииПубликация в OpenAlex
Аннотация: This study was conducted to identify and characterize dental follicle stem cells (DFSCs) by analyzing expression of embryonic, mesenchymal and neural stem cells surface markers. Design Dental follicle cells (DFCs) were evaluated by immunocytochemistry using embryonic stem cells markers (OCT4 and SOX2), mesenchmal stem cells (MSCs) markers (Notch1, active Notch1, STRO, CD44, HLA-ABC, CD90), neural stem cells markers (Nestin and β-III-tubulin), neural crest stem cells (NCSCs) markers (p75 and HNK1) and a glial cells marker (GFAP). RT-PCR was performed to identify the expression of OCT4 and NANOG in DFCs and dental follicle tissue.Immunocytochemistry and RT-PCR analysis revealed that a significant proportion of the DFCs evaluated expressed human embryonic stem cells marker OCT4 (75%) whereas NANOG was weakly expressed. A considerable amount of MSCs (90%) expressed Notch1, STRO, CD44 and HLA-ABC. However, they were weakly positive for CD90. Moreover, it was possible to demonstrate that dental follicle contains a significant proportion of neural stem/progenitors cells, expressing β-III-tubulin (90%) and nestin (70%). Interestingly, immunocytochemistry showed DFCs positive for p75 (50%), HNK1 (<10%) and a small proportion (<20%) of GFAP-positive cells. This is the first study reporting the presence of NCSCs and glial-like cells in the dental follicle.The results of the present study suggest the occurrence of heterogeneous populations of stem cells, particularly neural stem/progenitor cells, in the dental follicle, Therefore, the human dental follicle might be a promising source of adult stem cells for regenerative purposes.
Год издания: 2016
Авторы: Rodrigo Lopes de Lima, Rosenilde C. Holanda-Afonso, Vivaldo Moura‐Neto, Ana Maria Bolognese, Marcos F. DosSantos, Margareth Maria Gomes de Souza
Издательство: Elsevier BV
Источник: Archives of Oral Biology
Ключевые слова: Mesenchymal stem cell research, Cleft Lip and Palate Research, Hedgehog Signaling Pathway Studies
Другие ссылки: Archives of Oral Biology (HTML)
PubMed (HTML)