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Metabolic engineeringof Escherichia coliW3110 to produceL‐malate Xiaoxiang DongXiulai ChenYuanyuan Qian2016 год

Metabolic engineering of Escherichia coli W3110 to produce L‐malate
статья из журнала

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Аннотация: A four-carbon dicarboxylic acid L-malate has recently attracted attention due to its potential applications in the fields of medicine and agriculture. In this study, Escherichia coli W3110 was engineered and optimized for L-malate production via one-step L-malate synthesis pathway. First, deletion of the genes encoding lactate dehydrogenase (ldhA), pyruvate oxidase (poxB), pyruvate formate lyase (pflB), phosphotransacetylase (pta), and acetate kinase A (ackA) in pta-ackA pathway led to accumulate 20.9 g/L pyruvate. Then, overexpression of NADP+ -dependent malic enzyme C490S mutant in this multi-deletion mutant resulted in the direct conversion of pyruvate into L-malate (3.62 g/L). Next, deletion of the genes responsible for succinate biosynthesis further enhanced L-malate production up to 7.78 g/L. Finally, L-malate production was elevated to 21.65 g/L with the L-malate yield to 0.36 g/g in a 5 L bioreactor by overexpressing the pos5 gene encoding NADH kinase in the engineered E. coli F0931 strain. This study demonstrates the potential utility of one-step pathway for efficient L-malate production. Biotechnol. Bioeng. 2017;114: 656-664. © 2016 Wiley Periodicals, Inc.
Год издания: 2016
Авторы: Xiaoxiang Dong, Xiulai Chen, Yuanyuan Qian, Yuancai Wang, Li Wang, Weihua Qiao, Li Liu
Издательство: Wiley
Источник: Biotechnology and Bioengineering
Ключевые слова: Microbial Metabolic Engineering and Bioproduction, Enzyme Structure and Function, Bacterial Genetics and Biotechnology
Другие ссылки: Biotechnology and Bioengineering (HTML)
PubMed (HTML)