1. Главная
  2. Ресурсы
  3. Библиотечный поиск
NLRP3 inflammasome asa target of berberinein experimentalmurine liver injury:interference withP2X7 signalling Elisa VivoliA. CapponStefano Milani2016 год

NLRP3 inflammasome as a target of berberine in experimental murine liver injury: interference with P2X7 signalling
статья из журнала

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: Berberine (BRB) is commonly used in herbal medicine, but its mechanisms of action are poorly understood. In the present study, we tested BRB in steatohepatitis induced by a methionine- and choline-deficient (MCD) diet, in acute acetaminophen intoxication and in cultured murine macrophages. BRB markedly improved parameters of liver injury and necroinflammation induced by the MCD diet, although increased mortality was observed by mechanisms independent of bacterial infections or plasma levels of BRB. The MCD diet induced up-regulation of all components of the NLRP3 (NACHT, LRR and PYD domain-containing protein 3) inflammasome, and increased hepatic levels of mature IL-1β (interleukin 1β). All of these parameters were significantly reduced in mice treated with BRB. In mice administered an acetaminophen overdose, a model dependent on inflammasome activation, BRB reduced mortality and ALT (alanine aminotransferase) elevation, and limited the expression of inflammasome components. In vitro, LPS (lipopolysaccharide)-induced activation of NLRP3 inflammasome in RAW264.7 murine macrophages was markedly decreased by pre-incubation with BRB. BRB significantly limited the activation of the purinergic receptor P2X7, involved in the late phases of inflammasome activation. Upon P2X7 knockdown, the ability of BRB to block LPS-induced secretion of IL-1β was lost. These data indicate that administration of BRB ameliorates inflammation and injury in two unrelated murine models of liver damage. We demonstrate for the first time that BRB interferes with activation of the NLRP3 inflammasome pathway in vivo and in vitro, through a mechanism based on interference with activation of P2X7, a purinergic receptor involved in inflammasome activation.
Год издания: 2016
Авторы: Elisa Vivoli, A. Cappon, Stefano Milani, Benedetta Piombanti, A. Provenzano, Erica Novo, Alessio Masi, Nadia Navari, Roberto Narducci, Guido Mannaioni, Gloriano Moneti, Cláudia P. Oliveira, Maurizio Parola, Fabio Marra
Издательство: Portland Press
Источник: Clinical Science
Ключевые слова: Berberine and alkaloids research, Cannabis and Cannabinoid Research, Neonatal Health and Biochemistry
Другие ссылки: Clinical Science (PDF)
Clinical Science (HTML)
Institutional Research Information System University of Turin (University of Turin) (PDF)
Institutional Research Information System University of Turin (University of Turin) (HTML)
Florence Research (University of Florence) (PDF)
Florence Research (University of Florence) (HTML)
PubMed (HTML)