1. Главная
  2. Ресурсы
  3. Библиотечный поиск
Cardiotonic PillReduces MyocardialIschemia-ReperfusionInjury via IncreasingEET Concentrations inRats Min XuHaiping HaoLifeng Jiang2016 год

Cardiotonic Pill Reduces Myocardial Ischemia-Reperfusion Injury via Increasing EET Concentrations in Rats
статья из журнала

Страница публикацииПубликация в OpenAlex
Аннотация: Accumulating data suggest that epoxyeicosatrienoic acids (EETs) and 20-hydroxyeicosatetraenoic acid, both cytochrome P450 (P450) enzyme metabolites of arachidonic acid (AA), play important roles in cardiovascular diseases. For many years, the cardiotonic pill (CP), an herbal preparation derived from Salviae Miltiorrhizae Radix et Rhizoma, Notoginseng Radix et Rhizoma, and Borneolum Syntheticum, has been widely used in China for the treatment of coronary artery disease. However, its pharmacological mechanism has not been well elucidated. The purpose of this study was to investigate the chronic effects of the CP on myocardial ischemia-reperfusion injury (MIRI) and AA P450 enzyme metabolism in rats (in vivo) and H9c2 cells (in vitro). The results showed that CP dose dependently (10, 20, and 40 mg/kg/d; 7 days) mitigated MIRI in rats. The plasma concentrations of EETs in CP-treated ischemia-reperfusion (I/R) rats (40 mg/kg/d; 7 days) were significantly higher (P < 0.05) than those in controls. Cardiac Cyp1b1, Cyp2b1, Cyp2e1, Cyp2j3, and Cyp4f6 were significantly induced (P < 0.05); CYP2J and CYP2C11 proteins were upregulated (P < 0.05); and AA-epoxygenases activity was significantly increased (P < 0.05) after CP (40 mg/kg/d; 7 days) administration in rats. In H9c2 cells, the CP also increased (P < 0.05) the EET concentrations and showed protection in hypoxia-reoxygenation (H/R) cells. However, an antagonist of EETs, 14,15-epoxyeicosa-5(Z)-enoic acid, displayed a dose-dependent depression of the CP's protective effects in H/R cells. In conclusion, upregulation of cardiac epoxygenases after multiple doses of the CP—leading to elevated concentrations of cardioprotective EETs after myocardial I/R—may be the underlying mechanism, at least in part, for the CP's cardioprotective effect in rats.
Год издания: 2016
Авторы: Min Xu, Haiping Hao, Lifeng Jiang, Yingjie Wei, Fengbiao Zhou, Jianguo Sun, Jing Zhang, Hui Ji, Gang Wang, Wenzheng Ju, Puying Li
Издательство: American Society for Pharmacology and Experimental Therapeutics
Источник: Drug Metabolism and Disposition
Ключевые слова: Eicosanoids and Hypertension Pharmacology, Hormonal Regulation and Hypertension, Free Radicals and Antioxidants
Другие ссылки: Drug Metabolism and Disposition (HTML)
PubMed (HTML)