Use of DNA metabarcoding for stomach content analysis in the invasive lionfish Pterois volitans in Puerto Ricoстатья из журнала
Аннотация: MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 558:181-191 (2016) - DOI: https://doi.org/10.3354/meps11738 Use of DNA metabarcoding for stomach content analysis in the invasive lionfish Pterois volitans in Puerto Rico Chelsea A. Harms-Tuohy*, Nikolaos V. Schizas, Richard S. Appeldoorn Department of Marine Sciences, University of Puerto Rico at Mayagüez, Call Box 9000, Mayagüez, PR 00681, USA *Corresponding author: chelsea.harms@upr.edu ABSTRACT: Studies of lionfish feeding ecology seek to document the ecological impact of this invasive predatory species and determine which native prey species are at greatest risk. There are 2 common approaches to feeding ecology through gut content analysis: morphological identification to the lowest possible taxonomic rank and/or DNA barcoding of individual prey components in the stomach. The major disadvantage of both techniques is their inability to use advanced digested material. This study introduces next-generation sequencing to lionfish feeding ecology, employing DNA metabarcoding to analyze all components of the gut contents, including the previously unidentifiable portion. Sixty-three lionfish were caught from the inshore and offshore reefs of La Parguera, Puerto Rico. Stomach contents were separated into 2 sample components—a liquid (i.e. digested) and undigested tissue. A 313 bp region of the cytochrome oxidase subunit I (COI) gene was amplified from extracted DNA using specific primers for Caribbean reef fish. Samples were sequenced with an Illumina MiSeq platform, and the resulting 950+ sequences were compared against GenBank and the Barcode of Life Database to identify specimens at the lowest taxonomic level. Thirty-nine fish species from 16 families were identified (35 each in the digested and tissue fractions), including members of Pomacentridae, Acanthuridae, Gobiidae, Apogonidae, and Scaridae. Using the digested liquiform material proved efficient in detecting prey species, especially those that would have been missed with traditional methods. KEY WORDS: Reef fish · Feeding ecology · Invasive species · Caribbean · Cytochrome oxidase subunit I · COI · Next-generation sequencing · NGS Full text in pdf format Supplementary material PreviousNextCite this article as: Harms-Tuohy CA, Schizas NV, Appeldoorn RS (2016) Use of DNA metabarcoding for stomach content analysis in the invasive lionfish Pterois volitans in Puerto Rico. Mar Ecol Prog Ser 558:181-191. https://doi.org/10.3354/meps11738 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 558. Online publication date: October 25, 2016 Print ISSN: 0171-8630; Online ISSN: 1616-1599 Copyright © 2016 Inter-Research.
Год издания: 2016
Авторы: CA Harms-Tuohy, NV Schizas, Richard S. Appeldoorn
Издательство: Inter-Research
Источник: Marine Ecology Progress Series
Ключевые слова: Marine Ecology and Invasive Species, Environmental DNA in Biodiversity Studies, Identification and Quantification in Food
Другие ссылки: Marine Ecology Progress Series (PDF)
Marine Ecology Progress Series (HTML)
NOAA Institutional Repository (PDF)
NOAA Institutional Repository (HTML)
Marine Ecology Progress Series (HTML)
NOAA Institutional Repository (PDF)
NOAA Institutional Repository (HTML)
Открытый доступ: bronze
Том: 558
Страницы: 181–191