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Surfactant tuning ofhydrophilicity ofporous degradablecopolymer scaffoldspromotes cellularproliferation andenhances boneformation Mohammed A. YassinKnut N. LeknesYang Sun2016 год

Surfactant tuning of hydrophilicity of porous degradable copolymer scaffolds promotes cellular proliferation and enhances bone formation
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Аннотация: Poly(l-lactide-co-ɛ-caprolactone) (poly(LLA-co-CL)) has been blended with Tween 80 to tune the material properties and optimize cell-material interactions. Accordingly, the aims of this study were fourfold: to evaluate the effect of low concentrations of Tween 80 on the surface microstructure of 3D poly(LLA-co-CL) porous scaffolds: to determine the effect of different concentrations of Tween 80 on proliferation of bone marrow stromal cells (BMSCs) in vitro under dynamic cell culture at 7 and 21 days; to assess the influence of Tween 80 on the degradation rate of poly(LLA-co-CL) at 7 and 21 days; and in a subcutaneous rat model, to evaluate the effect on bone formation of porous scaffolds modified with 3% Tween 80 at 2 and 8 weeks. Blending 3% (w/w) Tween 80 with poly(LLA-co-CL) improves the surface wettability (p < 0.001). Poly(LLA-co-CL)/3% Tween 80 shows significantly increased cellular proliferation at days 7 and 21 (p < 0.001). Moreover, the presence of Tween 80 facilitates the degradation of poly(LLA-co-CL). Two weeks post-implantation, the poly(LLA-co-CL)/3% Tween 80 scaffolds exhibit significant mRNA expression of Runx2 (p = 0.004). After 8 weeks, poly(LLA-co-CL)/3% Tween 80 scaffolds show significantly increased de novo bone formation, demonstrated by μ-CT (p = 0.0133) and confirmed histologically. It can be concluded that blending 3% (w/w) Tween 80 with poly (LLA-co-CL) improves the hydrophilicity and osteogenic potential of the scaffolds. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2049-2059, 2016.
Год издания: 2016
Авторы: Mohammed A. Yassin, Knut N. Leknes, Yang Sun, Stein Atle Lie, Anna Finne‐Wistrand, Kamal Mustafa
Издательство: Wiley
Источник: Journal of Biomedical Materials Research Part A
Ключевые слова: Bone Tissue Engineering Materials, biodegradable polymer synthesis and properties, Orthopaedic implants and arthroplasty
Другие ссылки: Journal of Biomedical Materials Research Part A (HTML)
Bergen Open Research Archive (BORA) (University of Bergen) (PDF)
Bergen Open Research Archive (BORA) (University of Bergen) (HTML)
PubMed (HTML)