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Metabolismofα-linolenic acidduring incubationswith strained bovinerumen contents:products andmechanisms Anne M. HonkanenHeidi LeskinenVesa Toivonen2016 год

Metabolism ofα-linolenic acid during incubations with strained bovine rumen contents: products and mechanisms
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Аннотация: Description of α-linolenic acid (cis-9,cis-12,cis-15-18 : 3, ALA) metabolism in the rumen is incomplete. Ruminal digesta samples were incubated with ALA and buffer containing water or deuterium oxide to investigate the products and mechanisms of ALA biohydrogenation. Geometric Δ9,11,15-18 : 3 isomers were the main intermediates formed from ALA. An increase in the n+1 isotopomers of Δ9,11,15-18 : 3 was due to 2H labelling at C-13. Isomers of Δ9,11,13-18 : 3, cis-7,cis-12,cis-15-18 : 3 and cis-8,cis-12,cis-15-18 : 3 were also formed. No increase in n+1 isotopomers of Δ7,12,15-18 : 3 or Δ8,12,15-18 : 3 was detected. Enrichment in n+2 isotopomers of 18 : 2 products indicated that ALA metabolism continued via the reduction of 18 : 3 intermediates. Isomers of Δ9,11,15-18 : 3 were reduced to Δ11,15-18 : 2 labelled at C-9 and C-13. ALA resulted in the formation of Δ11,13-18 : 2 and Δ12,14-18 : 2 containing multiple 2H labels. Enrichment of the n+3 isotopomer of Δ12,15-18 : 2 was also detected. Metabolism of ALA during incubations with rumen contents occurs by one of three distinct pathways. Formation of Δ9,11,15-18 : 3 appears to be initiated by H abstraction on C-13. Octadecatrienoic intermediates containing cis-12 and cis-15 double bonds are formed without an apparent H exchange with water. Labelling of Δ9,11,13-18 : 3 was inconclusive, suggesting formation by an alternative mechanism. These findings explain the appearance of several bioactive fatty acids in muscle and milk that influence the nutritional value of ruminant-derived foods.
Год издания: 2016
Авторы: Anne M. Honkanen, Heidi Leskinen, Vesa Toivonen, Nest McKain, R. J. Wallace, K.J. Shingfield
Издательство: Cambridge University Press
Источник: British Journal Of Nutrition
Ключевые слова: Fatty Acid Research and Health, Lipid metabolism and biosynthesis, Ruminant Nutrition and Digestive Physiology
Другие ссылки: British Journal Of Nutrition (PDF)
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Aberystwyth Research portal (Aberystwyth University) (HTML)
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Aberystwyth Research portal (Aberystwyth University) (HTML)
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