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Metabarcoding ofmarine zooplankton:prospects, progressand pitfalls Ann BucklinPenelope K. LindequeNaiara Rodríguez‐Ezpeleta2016 год

Metabarcoding of marine zooplankton: prospects, progress and pitfalls
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Аннотация: Metabarcoding (large-scale taxonomic identification of complex samples via analysis of one or few orthologous DNA regions, called barcodes) is revolutionizing analysis of biodiversity of marine zooplankton assemblages. Metabarcoding relies on high-throughput DNA sequencing (HTS) technologies, which yield millions of DNA sequences in parallel and allow large-scale analysis of environmental samples. Metabarcoding studies of marine zooplankton have used various regions of nuclear small- (18S) and large-subunit (28S) rRNA, which allow accurate classification of novel sequences and reliable amplification with consensus primers, but- due to their relatively conserved nature- may underestimate species diversity in a community. To discriminate species, more variable genes are needed. A limited number of metabarcoding studies have used mitochondrial cytochrome oxidase I (COI), which ensures detection of species-level diversity, but may require group-specific primers and thus result in inconsistent amplification success rates. Reference databases with sequences for accurately-identified species are critically needed to allow taxonomic designation of molecular operational taxonomic units (MOTU) and comparison with previous studies of zooplankton diversity. Potential and promising applications of metabarcoding include rapid detection of impacts of climate change, monitoring and assessment of ecosystem health, calculation of biotic indices, characterization of food webs and detection of introduced, non-indigenous species.
Год издания: 2016
Авторы: Ann Bucklin, Penelope K. Lindeque, Naiara Rodríguez‐Ezpeleta, Aitor Albaina, Maiju Lehtiniemi
Издательство: Oxford University Press
Источник: Journal of Plankton Research
Ключевые слова: Environmental DNA in Biodiversity Studies, Identification and Quantification in Food, Microbial Community Ecology and Physiology
Другие ссылки: Journal of Plankton Research (PDF)
Journal of Plankton Research (HTML)
Zenodo (CERN European Organization for Nuclear Research) (PDF)
Zenodo (CERN European Organization for Nuclear Research) (HTML)
Plymouth Marine Science Electronic Archive (The Marine Biological Association (MBA), Plymouth Marine Laboratory (PML) and the Sir Alister Hardy Foundation for Ocean Science (SAHFOS).) (HTML)