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Reproducibility ofFluorescentExpression fromEngineered BiologicalConstructs in E. coli Jacob BealTraci Haddock-AngelliMarkus Gershater2016 год

Reproducibility of Fluorescent Expression from Engineered Biological Constructs in E. coli
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Аннотация: We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.
Год издания: 2016
Авторы: Jacob Beal, Traci Haddock-Angelli, Markus Gershater, Kim de Mora, Meagan Lizarazo, Jim Hollenhorst, Randy Rettberg
Издательство: Public Library of Science
Источник: PLoS ONE
Ключевые слова: Viral Infectious Diseases and Gene Expression in Insects, Gene Regulatory Network Analysis, CRISPR and Genetic Engineering
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