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MesodermaliPSC–derivedprogenitor cellsfunctionallyregenerate cardiacand skeletal muscle Mattia QuattrocelliMelissa SwinnenGiorgia Giacomazzi2015 год

Mesodermal iPSC–derived progenitor cells functionally regenerate cardiac and skeletal muscle
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Аннотация: Conditions such as muscular dystrophies (MDs) that affect both cardiac and skeletal muscles would benefit from therapeutic strategies that enable regeneration of both of these striated muscle types. Protocols have been developed to promote induced pluripotent stem cells (iPSCs) to differentiate toward cardiac or skeletal muscle; however, there are currently no strategies to simultaneously target both muscle types. Tissues exhibit specific epigenetic alterations; therefore, source-related lineage biases have the potential to improve iPSC-driven multilineage differentiation. Here, we determined that differential myogenic propensity influences the commitment of isogenic iPSCs and a specifically isolated pool of mesodermal iPSC-derived progenitors (MiPs) toward the striated muscle lineages. Differential myogenic propensity did not influence pluripotency, but did selectively enhance chimerism of MiP-derived tissue in both fetal and adult skeletal muscle. When injected into dystrophic mice, MiPs engrafted and repaired both skeletal and cardiac muscle, reducing functional defects. Similarly, engraftment into dystrophic mice of canine MiPs from dystrophic dogs that had undergone TALEN-mediated correction of the MD-associated mutation also resulted in functional striatal muscle regeneration. Moreover, human MiPs exhibited the same capacity for the dual differentiation observed in murine and canine MiPs. The findings of this study suggest that MiPs should be further explored for combined therapy of cardiac and skeletal muscles.
Год издания: 2015
Авторы: Mattia Quattrocelli, Melissa Swinnen, Giorgia Giacomazzi, J. Camps, Inès Barthélémy, Gabriele Ceccarelli, Ellen Caluwé, Hanne Grosemans, Lieven Thorrez, Glória Pelizzo, Manja Muijtjens, Catherine M. Verfaillie, Stéphane Blot, Stefan Janssens, Maurilio Sampaolesi
Издательство: American Society for Clinical Investigation
Источник: Journal of Clinical Investigation
Ключевые слова: Tissue Engineering and Regenerative Medicine, Muscle Physiology and Disorders, Pluripotent Stem Cells Research
Другие ссылки: Journal of Clinical Investigation (PDF)
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Europe PMC (PubMed Central) (PDF)
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Lirias (KU Leuven) (PDF)
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Archivio Istituzionale della Ricerca (Universita Degli Studi Di Milano) (PDF)
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IRIS Research product catalog (Sapienza University of Rome) (PDF)
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