Аннотация:RT–PCR‐southern hybridization analyses with radiolabelled P2Y receptor cDNAs as probes indicated that the peripheral blood leukocytes and the human umbilical vein endothelial cells express P2Y 1 , P2Y 2 , P2Y 4 and P2Y 6 receptors. Of the haematopoietic cell lines tested, promonocytic U937 cells express P2Y 2 and P2Y 6 , but not P2Y 1 or P2Y 4 ; promyelocytic HL‐60 cells express the P2Y 1 , P2Y 2 and P2Y 6 receptors but not the P2Y 4 receptor; K562 cells express P2Y 1 but not P2Y 2 , P2Y 4 or P2Y 6 ; and Dami cells express P2Y 1 , P2Y 2 , P2Y 4 and P2Y 6 receptors. Of the peripheral blood leukocytes tested, polymorphonuclear cells express P2Y 4 and P2Y 6 but not P2Y 1 or P2Y 2 receptors; monocytes express P2Y 1 , P2Y 2 , P2Y 4 and P2Y 6 receptors and lymphocytes express P2Y 1 , P2Y 2 , P2Y 4 and P2Y 6 receptors. These results suggest a physiological role for different P2Y receptor subtypes in the extracellular nucleotide‐mediated stimulation of monocytes, neutrophils, lymphocytes and endothelial cells. British Journal of Pharmacology (1998) 123 , 789–794; doi: 10.1038/sj.bjp.0701665
