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Identification anddetermination of themajor constituents intraditional Chinesemedicinal plantPolygonum multiflorumthunb by HPLC coupledwith PAD… Tao YiKelvin Sze‐Yin LeungGuanghua Lu2007 год

Identification and determination of the major constituents in traditional Chinese medicinal plant Polygonum multiflorum thunb by HPLC coupled with PAD and ESI/MS
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Аннотация: Abstract An HPLC–PAD–MS method has been developed for the qualitative and quantitative analysis of the major chemical constituents in Polygonum multiflorum Thunb. Chromatographic separation was conducted on an Alltima C 18 column using water:acetonitrile:acetic acid as the mobile phase. Altogether nine compounds of various classes, including stilbene glucosides, anthraquinone glucosides and anthraquinone derivates, were identified by online ESI/MS. Their identities were ascertained by comparison with data derived from the literature and/or standard compounds. Five components were quantified by HPLC–PAD and the method was fully validated. All the linear regressions were acquired with R 2 > 0.99 and the quantification limits (with a signal‐to‐noise ratio of 3) ranged from 0.63 and 1.57 ng. Repeatability was evaluated by intra‐ and inter‐day assays and RSD value was within 2.47%. Recovery studies for the quantified compounds were found to be within the range 96.32–102.53% with RSD less than 2.35%. The overall procedure is rapid and reproducible and is considered suitable for qualitative and quantitative analysis of a large number of samples. Copyright © 2007 John Wiley & Sons, Ltd.
Год издания: 2007
Авторы: Tao Yi, Kelvin Sze‐Yin Leung, Guanghua Lu, Hao Zhang, Kelvin Chan
Издательство: Wiley
Источник: Phytochemical Analysis
Ключевые слова: Phytochemistry and biological activity of medicinal plants, Natural product bioactivities and synthesis, Phytochemistry and Biological Activities
Другие ссылки: Phytochemical Analysis (HTML)
PubMed (HTML)