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Chemical Methods forPeptide and ProteinProduction Saranya ChandruduPavla SimerskáIstván Tóth2013 год

Chemical Methods for Peptide and Protein Production
review

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: Since the invention of solid phase synthetic methods by Merrifield in 1963, the number of research groups focusing on peptide synthesis has grown exponentially. However, the original step-by-step synthesis had limitations: the purity of the final product decreased with the number of coupling steps. After the development of Boc and Fmoc protecting groups, novel amino acid protecting groups and new techniques were introduced to provide high quality and quantity peptide products. Fragment condensation was a popular method for peptide production in the 1980s, but unfortunately the rate of racemization and reaction difficulties proved less than ideal. Kent and co-workers revolutionized peptide coupling by introducing the chemoselective reaction of unprotected peptides, called native chemical ligation. Subsequently, research has focused on the development of novel ligating techniques including the famous click reaction, ligation of peptide hydrazides, and the recently reported a-ketoacid-hydroxylamine ligations with 5-oxaproline. Several companies have been formed all over the world to prepare high quality Good Manufacturing Practice peptide products on a multi-kilogram scale. This review describes the advances in peptide chemistry including the variety of synthetic peptide methods currently available and the broad application of peptides in medicinal chemistry.
Год издания: 2013
Авторы: Saranya Chandrudu, Pavla Simerská, István Tóth
Издательство: Multidisciplinary Digital Publishing Institute
Источник: Molecules
Ключевые слова: Chemical Synthesis and Analysis, Click Chemistry and Applications, Biochemical and Structural Characterization
Другие ссылки: Molecules (PDF)
Molecules (HTML)
DOAJ (DOAJ: Directory of Open Access Journals) (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
CiteSeer X (The Pennsylvania State University) (PDF)
CiteSeer X (The Pennsylvania State University) (HTML)
PubMed (HTML)