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Regulation of cellsubstrate adhesion:effects of smallgalactosaminoglycan-containingproteoglycans. Deborah J. BidansetRichard G. LeBaronLynn Rosenberg1992 год

Regulation of cell substrate adhesion: effects of small galactosaminoglycan-containing proteoglycans.
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Аннотация: Cell adhesion is a process which is initiated by the attachment of cells to specific sites in adhesive matrix proteins via cell surface receptors of the integrin family. This is followed by a reorganization of cytoskeletal elements which results in cell spreading and the formation of focal adhesion plaques. We have examined the effects of a class of small galactosaminoglycan-containing proteoglycans on the various stages of cell adhesion to fibronectin-coated substrates. Our results indicate that dermatan sulfate proteoglycans (DSPGs) derived from cartilage, as well as other related small proteoglycans, inhibit the initial attachment of CHO cells and rat embryo fibroblasts to substrates composed of the 105-kD cell-binding fibronectin fragment, but do not affect cell attachment to intact fibronectin. Although this effect involves binding of DSPGs to the substrate via the protein core, the intact proteoglycan is necessary for the observed activity. Isolated core proteins are inactive. The structural composition of the galactosaminoglycan chain does not appear to be functionally significant since both chondroitin sulfate and various dermatan sulfate proteoglycans of this family inhibit cell attachment to the fibronectin fragment. Neither the percentage of cells spread nor the mean area of spread cells adhering to substrates of intact fibronectin was significantly affected by the DSPGs. However, significantly fewer cells formed focal adhesions in the presence of DSPGs as compared with untreated control cells. These results suggest that the binding of small galactosaminoglycan-containing proteoglycans to a fibronectin substrate may affect several stages in the cell adhesion process.
Год издания: 1992
Авторы: Deborah J. Bidanset, Richard G. LeBaron, Lynn Rosenberg, Joanne E. Murphy-Ullrich, Magnus Höök
Издательство: Rockefeller University Press
Источник: The Journal of Cell Biology
Ключевые слова: Proteoglycans and glycosaminoglycans research, Cell Adhesion Molecules Research, Glycosylation and Glycoproteins Research
Другие ссылки: The Journal of Cell Biology (PDF)
The Journal of Cell Biology (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)