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Coupling of thenucleus andcytoplasm: Role ofthe LINC complex Melissa CrispQian LiuKyle J. Roux2005 год

Coupling of the nucleus and cytoplasm: Role of the LINC complex
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Аннотация: The nuclear envelope defines the barrier between the nucleus and cytoplasm and features inner and outer membranes separated by a perinuclear space (PNS). The inner nuclear membrane contains specific integral proteins that include Sun1 and Sun2. Although the outer nuclear membrane (ONM) is continuous with the endoplasmic reticulum, it is nevertheless enriched in several integral membrane proteins, including nesprin 2 Giant (nesp2G), an 800-kD protein featuring an NH2-terminal actin-binding domain. A recent study (Padmakumar, V.C., T. Libotte, W. Lu, H. Zaim, S. Abraham, A.A. Noegel, J. Gotzmann, R. Foisner, and I. Karakesisoglou. 2005. J. Cell Sci. 118:3419–3430) has shown that localization of nesp2G to the ONM is dependent upon an interaction with Sun1. In this study, we confirm and extend these results by demonstrating that both Sun1 and Sun2 contribute to nesp2G localization. Codepletion of both of these proteins in HeLa cells leads to the loss of ONM-associated nesp2G, as does overexpression of the Sun1 lumenal domain. Both treatments result in the expansion of the PNS. These data, together with those of Padmakumar et al. (2005), support a model in which Sun proteins tether nesprins in the ONM via interactions spanning the PNS. In this way, Sun proteins and nesprins form a complex that links the nucleoskeleton and cytoskeleton (the LINC complex).
Год издания: 2005
Авторы: Melissa Crisp, Qian Liu, Kyle J. Roux, J. B. Rattner, Catherine M. Shanahan, Brian Burke, Phillip D. Stahl, Didier Hodzic
Издательство: Rockefeller University Press
Источник: The Journal of Cell Biology
Ключевые слова: Nuclear Structure and Function, RNA Research and Splicing, RNA regulation and disease
Другие ссылки: The Journal of Cell Biology (PDF)
The Journal of Cell Biology (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
durham-repository.worktribe.com (HTML)
PubMed Central (HTML)
Durham Research Online (Durham University) (PDF)
Durham Research Online (Durham University) (HTML)
PubMed (HTML)