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Response ofosteoblast‐likeSAOS‐2 cells tozirconia ceramicswith differentsurface topographies Ute HempelThomas HeftiMarie Hubálek Kalbáčová2009 год

Response of osteoblast‐like SAOS‐2 cells to zirconia ceramics with different surface topographies
статья из журнала

Страница публикацииПубликация в OpenAlex
Аннотация: Zirconia is a suitable biomaterial for use in medicine (stomatology, orthopaedics) due to its good biocompatibility and outstanding mechanical properties. This study compares the effect of (i) zirconia to the widely used titanium and (ii) zirconia with two different surface topographies (sandblasted and sandblasted/etched) on the adhesion, proliferation and differentiation of SAOS-2 osteoblasts.SAOS-2 cells were cultured on either sandblasted or sandblasted/etched zirconia and compared with sandblasted/etched titanium. 2 and 24 h after plating, cell morphology was investigated by scanning electron microscope (SEM) and fluorescence imaging. At 24 and 48 h, cell number-relevant parameters were determined. Alkaline phosphatase (ALP) activity and mineral accumulation were measured at days 8, 11, 15 and day 22 of culture, respectively.SEM and fluorescence images revealed a faster spreading as well as higher number of adherent cells after 24 h incubation on zirconia compared with titanium. Also, the cellular metabolic activity after 24 h and the proliferation rate after 48 h is higher with zirconia compared with titanium. Zirconia had a more pronounced effect compared with titanium on the differentiation of SAOS-2 cells: ALP activity, an early differentiation marker increased earlier and mineralization, a late differentiation marker was increased. Only minor differences were found between zirconia with two different surface topographies; etched zirconia promoted slightly greater the differentiation of SAOS-2 cells.These data indicate that zirconia mediates a pronounced stronger effect on the adhesion, proliferation and differentiation compared with titanium; and that topographical differences of zirconia have minor effects on osteoblast biology.
Год издания: 2009
Авторы: Ute Hempel, Thomas Hefti, Marie Hubálek Kalbáčová, Cornelia Wolf‐Brandstetter, Peter Dieter, Falko Schlottig
Издательство: Wiley
Источник: Clinical Oral Implants Research
Ключевые слова: Bone Tissue Engineering Materials, Dental materials and restorations, Dental Implant Techniques and Outcomes
Другие ссылки: Clinical Oral Implants Research (HTML)
PubMed (HTML)