P4–324: Periodontitis induced by bacterial infection exacerbates features of Alzheimer's disease in transgenic miceстатья из журнала
Аннотация: Periodontitis is a localized infectious disease caused by periodontopathic bacteria such as Porphyromonas gingivalis (P. gingivalis). Recently, it has been reported that periodontitis is associated with systemic diseases such as diabetes and atherosclerosis. It has been also suggested that bacterial infections may contribute to the onset and the progression of Alzheimer's disease (AD). However, we do not have any evidence about the causative relationship between periodontitis and AD. Herein, we explore whether periodontitis evoked by P. gingivalis modulates the pathological features of AD using a transgenic mouse model of AD. Periodontitis was induced in J20 mice by inoculations with P. gingivalis for five weeks and evaluated by micro-computed tomography measurements of bone loss. We performed passive avoidance tests and novel objection tests on these mice to assess AD-related behavioral functions. Next, we measured the levels of amyloid beta - protein (A beta)1–40 and A beta 1–42 in the soluble and insoluble fractions from brain samples of the mice using an ELISA. In addition, we performed immunohistochemical analysis to examine the brain A beta deposition in these mice. We also measured the levels of IL-1 beta and TNF -alpha in the brain samples to evaluate inflammation in the brain. We found that cognitive functions were significantly impaired in periodontitis-developed J20 mice, compared to control J20 mice that were not inoculated. Levels of A beta 1–40 and A beta 1–42 in the brain were also higher in inoculated J20 mice than in control J20 mice. Levels of A beta deposition in both hippocampus and cortex were higher in inoculated J20 mice than in control J20 mice. Furthermore, the level of proinflammatory cytokines, such as IL-1 beta and TNF-alpha, in the brain were higher in inoculated mice than in control mice. These results suggest that periodontitis evoked by P. gingivalis may exacerbate brain amyloid deposition, leading to enhanced cognitive impairments, by a mechanism that involves triggering brain inflammation.
Год издания: 2013
Авторы: Naoyuki Ishida, Yuichi Ishihara, Kazuto Ishida, Hiroyuki Tada, Yoshiko Kato, Ryutarou Isoda, Makoto Hagiwara, Makoto Michikawa, Toshihide Noguchi, Kenji Matsushita
Издательство: Wiley
Источник: Alzheimer s & Dementia
Ключевые слова: Alzheimer's disease research and treatments, S100 Proteins and Annexins, Oral microbiology and periodontitis research
Открытый доступ: closed
Том: 9
Выпуск: 4S_Part_21