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A Role for AutophagicProtein Beclin 1Early in LymphocyteDevelopment Ivica ArsovAdeola AdebayoMartina Kučerová-Levisohn2011 год

A Role for Autophagic Protein Beclin 1 Early in Lymphocyte Development
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Аннотация: Abstract Autophagy is a highly regulated and evolutionarily conserved process of cellular self-digestion. Recent evidence suggests that this process plays an important role in regulating T cell homeostasis. In this study, we used Rag1−/− (recombination activating gene 1−/−) blastocyst complementation and in vitro embryonic stem cell differentiation to address the role of Beclin 1, one of the key autophagic proteins, in lymphocyte development. Beclin 1-deficient Rag1−/− chimeras displayed a dramatic reduction in thymic cellularity compared with control mice. Using embryonic stem cell differentiation in vitro, we found that the inability to maintain normal thymic cellularity is likely caused by impaired maintenance of thymocyte progenitors. Interestingly, despite drastically reduced thymocyte numbers, the peripheral T cell compartment of Beclin 1-deficient Rag1−/− chimeras is largely normal. Peripheral T cells displayed normal in vitro proliferation despite significantly reduced numbers of autophagosomes. In addition, these chimeras had greatly reduced numbers of early B cells in the bone marrow compared with controls. However, the peripheral B cell compartment was not dramatically impacted by Beclin 1 deficiency. Collectively, our results suggest that Beclin 1 is required for maintenance of undifferentiated/early lymphocyte progenitor populations. In contrast, Beclin 1 is largely dispensable for the initial generation and function of the peripheral T and B cell compartments. This indicates that normal lymphocyte development involves Beclin 1-dependent, early-stage and distinct, Beclin 1-independent, late-stage processes.
Год издания: 2011
Авторы: Ivica Arsov, Adeola Adebayo, Martina Kučerová-Levisohn, Joanna E. Haye, Margaret A. MacNeil, F. Nina Papavasiliou, Zhenyu Yue, Benjamin Ortiz
Издательство: American Association of Immunologists
Источник: The Journal of Immunology
Ключевые слова: Autophagy in Disease and Therapy, Phagocytosis and Immune Regulation
Другие ссылки: The Journal of Immunology (PDF)
The Journal of Immunology (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)