Аннотация:Gene expression associated with soybean resistance to Piezodorus guildiniiSoybeans are an important crop with a world-wide annual production of 267.61 tons, worth 230 billion dollars.Stink bugs are a major soybean pest.The most abundant species in Brazil are Euschistus heros (E.), Piezodorus guildinii (West.)and Nezara viridula (L.).Stinkbug attack causes several problems, including delayed physiological maturity, leaf retention, yield losses, decreased seed quality, and decreased germination potential.The bugs are responsible for transmitting pathogens, and they can cause changes in the oil, protein, and fatty acids composition in the seed.Producing genetically resistant cultivars is critical in order to minimize the need for agricultural pesticides.Our work aims to identify genes associated with the stink-bug resistance response in soybean by comparing differential gene expression between resistant (IAC-100) and susceptible (CD-215) genotypes by analyzing RNA sequencing (RNA-Seq) data.Data was from soybean pods that were subjected to two treatments for 24 hours: infestation by the stink bug Piezodorus guildinii (West.), and noninfestation.STAR aligner was used against the hard-masked genome sequence from Phytozome, followed by analysis using the Cufflinks package.Analysis of the results of the differentially expressed genes and a subsequent filtering identified 128 candidate defenseassociated genes, among them, defense-related such as LRR-RLKs and production of secondary metabolites like terpenes.
