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Designer deletionstrains derivedfromSaccharomycescerevisiae S288C: Auseful set of strainsand plasmids forPCR-mediated genedisruption and other… Carrie Baker BrachmannAdrian DaviesGregory J. Cost1998 год

Designer deletion strains derived fromSaccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications
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Аннотация: A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. Because the homology between commonly used auxotrophic marker gene segments and genomic sequences has been largely or completely abolished, these strains will also reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications. We also report the construction of new members of the pRS400 series of vectors, containing the kanMX, ADE2 and MET15 genes. © 1998 John Wiley & Sons, Ltd.
Год издания: 1998
Авторы: Carrie Baker Brachmann, Adrian Davies, Gregory J. Cost, Emerita M. Caputo, Joaquim Li, Philip Hieter, Jef D. Boeke
Издательство: Wiley
Источник: Yeast
Ключевые слова: Fungal and yeast genetics research, CRISPR and Genetic Engineering, RNA and protein synthesis mechanisms
Другие ссылки: Yeast (HTML)
PubMed (HTML)