1. Главная
  2. Ресурсы
  3. Библиотечный поиск
The TransmembraneSemaphorinSema4D/CD100, anInhibitor of AxonalGrowth, Is Expressedon Oligodendrocytesand Upregulated afterCNS Lesion Caroline Moreau‐FauvarqueAtsushi KumanogohEmeline Camand2003 год

The Transmembrane Semaphorin Sema4D/CD100, an Inhibitor of Axonal Growth, Is Expressed on Oligodendrocytes and Upregulated after CNS Lesion
статья из журнала

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: Semaphorins are a family of secreted and membrane-bound proteins, known to regulate axonal pathfinding. Sema4D, also called CD100, was first isolated in the immune system where it is involved in B and T cell activation. We found that in the mouse, Sema4D is expressed in cells throughout the CNS white matter, with a peak during the myelination period. Double-labeling experiments with different markers of oligodendrocyte lineage such as olig1, olig2, platelet-derived growth factor receptor alpha, and proteolipid protein showed that Sema4D was expressed selectively by oligodendrocytes and myelin. The presence of Sema4D in myelin was confirmed using Western blot. Sema4D expression in myelinating oligodendrocytes was further observed using neuron-oligodendrocyte cocultures. Moreover, using stripe assay, we found that Sema4D is strongly inhibitory for postnatal sensory and cerebellar granule cell axons. This prompted us to examine whether Sema4D expression is modified after CNS injury. At 8 d after spinal cord lesions, Sema4D expression was strongly upregulated in oligodendrocytes at the periphery of the lesion. Sema4D-positive cells were not colabeled with the astrocyte marker GFAP, with the microglial and macrophagic marker isolectin B4, or with NG2, a marker of oligodendrocyte precursors. This upregulation was transient because from 1 month after the lesion, Sema4D expression was back to its normal level. These results indicate that Sema4D is a novel inhibitory factor for axonal regeneration expressed in myelin.
Год издания: 2003
Авторы: Caroline Moreau‐Fauvarque, Atsushi Kumanogoh, Emeline Camand, Céline Jaillard, G. Barbin, Isabelle Boquet, Christopher Love, E. Yvonne Jones, Hitoshi Kikutani, Catherine Lubetzki, Isabelle Dusart, Alain Chédotal
Издательство: Society for Neuroscience
Источник: Journal of Neuroscience
Ключевые слова: Axon Guidance and Neuronal Signaling, Nerve injury and regeneration, Neurogenesis and neuroplasticity mechanisms
Другие ссылки: Journal of Neuroscience (PDF)
Journal of Neuroscience (HTML)
HAL (Le Centre pour la Communication Scientifique Directe) (HTML)
HAL (Le Centre pour la Communication Scientifique Directe) (HTML)
Griffith Research Online (Griffith University, Queensland, Australia) (PDF)
Griffith Research Online (Griffith University, Queensland, Australia) (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)