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Differential Effectsof Neurotrophins andSchwann Cell-DerivedSignals on NeuronalSurvival/Growth andSynaptogenesis H. Benjamin PengJiefei YangZhengshan Dai2003 год

Differential Effects of Neurotrophins and Schwann Cell-Derived Signals on Neuronal Survival/Growth and Synaptogenesis
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Аннотация: Recent studies have shown that the survival of mammalian motoneurons in vitro is promoted by neurotrophins (NTs) and cAMP. There is also evidence that neurotrophins enhance transmitter release. We thus investigated whether these agents also promote synaptogenesis. Cultured Xenopus spinal cord neurons were treated with a mixture of BDNF, glia-derived neurotrophic factor, NT-3, and NT-4, in addition to forskolin and IBMX or the cell-permeant form of cAMP, to elevate the cAMP level. The outgrowth and survival of neurons were dramatically increased by this trophic stimulation. However, when these neurons were cocultured with muscle cells, the trophic agents resulted in a failure of synaptogenesis. Specifically, the induction of ACh receptor (AChR) clustering in cultured muscle cells was inhibited at nerve-muscle contacts, in sharp contrast to control, untreated cocultures. Because AChR clustering induced by agrin or growth factor-coated beads in muscle cells was unaffected by trophic stimulation, its effect on synaptogenesis is presynaptic in origin. In the control, agrin was deposited along the neurite and at nerve-muscle contacts. This was significantly downregulated in cultures treated with trophic stimuli. Reverse transcriptase-PCR analyses showed that this decrease in agrin deposition was caused by an inhibition of agrin synthesis by trophic stimuli. Both agrin synthesis and induction of AChR clustering were restored under trophic stimulation when Schwann cell-conditioned medium was introduced. These results suggest that trophic stimulation maintains spinal neurons in the growth state, and Schwann cell-derived factors allow them to switch to the synaptogenic state.
Год издания: 2003
Авторы: H. Benjamin Peng, Jiefei Yang, Zhengshan Dai, Chi Wai Lee, Hiu Wai Hung, Zhi Hua Feng, Chien‐Ping Ko
Издательство: Society for Neuroscience
Источник: Journal of Neuroscience
Ключевые слова: Nerve injury and regeneration, Neurogenesis and neuroplasticity mechanisms, Signaling Pathways in Disease
Другие ссылки: Journal of Neuroscience (PDF)
Journal of Neuroscience (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
Carolina Digital Repository (University of North Carolina at Chapel Hill) (HTML)
PubMed Central (HTML)
PubMed (HTML)