Аннотация:Significance Many bacteria respond to environmental cues by producing cyclic diguanosine monophosphate (c-di-GMP), which binds to proteins to modulate transitions between sessile and motile lifestyles important for chronic and acute infections, respectively. The breadth of protein targets and mechanisms of action of c-di-GMP remain unknown. In the opportunistic pathogen Pseudomonas aeruginosa , c-di-GMP binds to FleQ, a transcription factor that is the master regulator of flagella synthesis and a member of the large family of AAA+ ATPase proteins. We found that c-di-GMP competitively inhibits FleQ ATPase activity and interacts with the ATP-binding site on FleQ. We suggest that AAA+ ATPases from other bacteria may respond to c-di-GMP, and that interference with ATPase activity is one mechanism of c-di-GMP action.
