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ICEPmu1, anintegrativeconjugative element(ICE) of Pasteurellamultocida: structureand transfer G. B. MichaelKristina KadlecM.T. Sweeney2011 год

ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer
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Страница публикацииПубликация в OpenAlex
Аннотация: Integrative and conjugative elements (ICEs) have not been detected in Pasteurella multocida. In this study the multiresistance ICEPmu1 from bovine P. multocida was analysed for its core genes and its ability to conjugatively transfer into strains of the same and different genera.ICEPmu1 was identified during whole genome sequencing. Coding sequences were predicted by bioinformatic tools and manually curated using the annotation software ERGO. Conjugation into P. multocida, Mannheimia haemolytica and Escherichia coli recipients was performed by mating assays. The presence of ICEPmu1 and its circular intermediate in the recipient strains was confirmed by PCR and sequence analysis. Integration sites were sequenced. Susceptibility testing of the ICEPmu1-carrying recipients was conducted by broth microdilution.The 82 214 bp ICEPmu1 harbours 88 genes. The core genes of ICEPmu1, which are involved in excision/integration and conjugative transfer, resemble those found in a 66 641 bp ICE from Histophilus somni. ICEPmu1 integrates into a tRNA(Leu) and is flanked by 13 bp direct repeats. It is able to conjugatively transfer to P. multocida, M. haemolytica and E. coli, where it also uses a tRNA(Leu) for integration and produces closely related 13 bp direct repeats. PCR assays and susceptibility testing confirmed the presence and the functional activity of the ICEPmu1-associated resistance genes in the recipient strains.The observation that the multiresistance ICEPmu1 is present in a bovine P. multocida and can easily spread across strain and genus boundaries underlines the risk of a rapid dissemination of multiple resistance genes, which will distinctly decrease the therapeutic options.
Год издания: 2011
Авторы: G. B. Michael, Kristina Kadlec, M.T. Sweeney, E. Brzuszkiewicz, Heiko Liesegang, Rolf Daniel, Robert W. Murray, Jeffrey L. Watts, S. Schwarz
Издательство: Oxford University Press
Источник: Journal of Antimicrobial Chemotherapy
Ключевые слова: Microbial infections and disease research, Plant Disease Resistance and Genetics, Plant Pathogenic Bacteria Studies
Другие ссылки: Journal of Antimicrobial Chemotherapy (HTML)
PubMed (HTML)