1. Главная
  2. Ресурсы
  3. Библиотечный поиск
TranscriptionalRepression by Msx-1Does Not RequireHomeodomainDNA-Binding Sites K M CatronHailan ZhangSally C. Marshall1995 год

Transcriptional Repression by Msx-1 Does Not Require Homeodomain DNA-Binding Sites
статья из журнала

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: AbstractThis study investigates the transcriptional properties of Msx-1, a murine homeodomain protein which has been proposed to play a key role in regulating the differentiation and/or proliferation state of specific cell populations during embryogenesis. We show, using basal and activated transcription templates, that Msx-1 is a potent repressor of transcription and can function through both TATA-containing and TATA-less promoters. Moreover, repression in vivo and in vitro occurs in the absence of DNA-binding sites for the Msx-1 homeodomain. Utilizing a series of truncated Msx-1 polypeptides, we show that multiple regions of Msx-1 contribute to repression, and these are rich in alanine, glycine, and proline residues. When fused to a heterologous DNA-binding domain, both N- and C-terminal regions of Msx-1 retain repressor function, which is dependent upon the presence of the heterologous DNA-binding site. Moreover, a polypeptide consisting of the full-length Msx-1 fused to a heterologous DNA-binding domain is a more potent repressor than either the N- or C-terminal regions alone, and this fusion retains the ability to repress transcription in the absence of the heterologous DNA site. We further show that Msx-1 represses transcription in vitro in a purified reconstituted assay system and interacts with protein complexes composed of TBP and TFIIA (DA) and TBP, TFIIA, and TFIIB (DAB) in gel retardation assays, suggesting that the mechanism of repression is mediated through interaction(s) with a component(s) of the core transcription complex. We speculate that the repressor function of Msx-1 is critical for its proposed role in embryogenesis as a regulator of cellular differentiation.
Год издания: 1995
Авторы: K M Catron, Hailan Zhang, Sally C. Marshall, Juan Inostroza, Jeanne Wilson, Cory Abate
Издательство: Taylor & Francis
Источник: Molecular and Cellular Biology
Ключевые слова: Developmental Biology and Gene Regulation, Animal Genetics and Reproduction, Genomics and Chromatin Dynamics
Другие ссылки: Molecular and Cellular Biology (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)