Comparative and functional genomic analyses of the pathogenicity of phytopathogenXanthomonas campestrispv.campestrisстатья из журнала
Аннотация: Xanthomonas campestris pathovar campestris (Xcc) is the causative agent of crucifer black rot disease, which causes severe losses in agricultural yield world-wide. This bacterium is a model organism for studying plant-bacteria interactions. We sequenced the complete genome of Xcc 8004 (5,148,708 bp), which is highly conserved relative to that of Xcc ATCC 33913. Comparative genomics analysis indicated that, in addition to a significant genomic-scale rearrangement cross the replication axis between two IS1478 elements, loss and acquisition of blocks of genes, rather than point mutations, constitute the main genetic variation between the two Xcc strains. Screening of a high-density transposon insertional mutant library (16,512 clones) of Xcc 8004 against a host plant (Brassica oleraceae) identified 75 nonredundant, single-copy insertions in protein-coding sequences (CDSs) and intergenic regions. In addition to known virulence factors, full virulence was found to require several additional metabolic pathways and regulatory systems, such as fatty acid degradation, type IV secretion system, cell signaling, and amino acids and nucleotide metabolism. Among the identified pathogenicity-related genes, three of unknown function were found in Xcc 8004-specific chromosomal segments, revealing a direct correlation between genomic dynamics and Xcc virulence. The present combination of comparative and functional genomic analyses provides valuable information about the genetic basis of Xcc pathogenicity, which may offer novel insight toward the development of efficient methods for prevention of this important plant disease.
Год издания: 2005
Авторы: Wei Qian, Yantao Jia, Shuangxi Ren, Yong-Qiang He, Jia‐Xun Feng, Ling-Feng Lu, Qi‐Hong Sun, Ying Ge, Dong‐Jie Tang, Hua Tang, Wei Wu, Pei Hao, Lifeng Wang, Bo‐Le Jiang, Shen-yan Zeng, Wenyi Gu, Gang Lü, Rong Li, Yingchuan Tian, Zhijian Yao, Gang Fu, Baoshan Chen, Rongxiang Fang, Boqin Qiang, Zhu Chen, Guoping Zhao, Ji‐Liang Tang, Chaozu He
Издательство: Cold Spring Harbor Laboratory Press
Источник: Genome Research
Ключевые слова: Plant Pathogenic Bacteria Studies, Plant-Microbe Interactions and Immunity, Legume Nitrogen Fixing Symbiosis
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Genome Research (HTML)
Europe PMC (PubMed Central) (PDF)
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Открытый доступ: hybrid
Том: 15
Выпуск: 6
Страницы: 757–767