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High expression ofleptin by human bonemarrow adipocytes inprimary culture Patrick LaharragueDominique LarrouyAnne-Marie Fontanilles1998 год

High expression of leptin by human bone marrow adipocytes in primary culture
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Аннотация: Adipocytes participate in the microenvironment of the bone marrow (BM), but their exact role remains to be determined. It has recently been shown that leptin, a hormone secreted from extramedullary adipocytes, could be involved in hematopoiesis. Therefore we have developed a primary culture system of human BM adipocytes to characterize their differentiation and determine whether leptin is also secreted from these adipocytes. BM cells were cultured with fetal calf and horse sera. In the presence of dexamethasone, cells with vesicles containing lipids appeared within 15 days. They expressed glycerol phosphate dehydrogenase activity and a lipolytic activity in response to isoproterenol, but expressed neither the adrenergic beta3 receptor nor the mitochondrial uncoupling protein UCP1. The addition of insulin alone to the culture media did not promote adipocyte differentiation. Leptin was expressed and secreted at high levels during adipocyte differentiation. Acute exposure of differentiated adipocytes to insulin had little effect on leptin expression whereas forskolin strongly inhibited it. These results show that although human BM adipocytes differ from extramedullary adipose tissues in their sensitivity to different effectors, they are a secondary source of leptin production. They suggest that BM adipocytes could contribute to hematopoiesis via the secretion of leptin in the vicinity of hematopoietic stem cells.
Год издания: 1998
Авторы: Patrick Laharrague, Dominique Larrouy, Anne-Marie Fontanilles, Nathalie Truel, A. Campfield, Renata Tenenbaum, Jean Galitzky, J Corberand, Luc Pénicaud, Louis Casteilla
Издательство: Wiley
Источник: The FASEB Journal
Ключевые слова: Regulation of Appetite and Obesity, Adipokines, Inflammation, and Metabolic Diseases, Adipose Tissue and Metabolism
Другие ссылки: The FASEB Journal (HTML)
HAL (Le Centre pour la Communication Scientifique Directe) (HTML)
HAL (Le Centre pour la Communication Scientifique Directe) (HTML)
PubMed (HTML)