How to assess Drosophila cold tolerance: chill coma temperature and lower lethal temperature are the best predictors of cold distribution limitsстатья из журнала
Аннотация: Summary Thermal tolerance may limit and therefore predict ectotherm geographic distributions. However, which of the many metrics of thermal tolerance best predict distribution is often unclear, even for drosophilids, which constitute a popular and well‐described animal model. Five metrics of cold tolerance were measured for 14 Drosophila species to determine which metrics most strongly correlate with geographic distribution. The species represent tropical to temperate regions but all were reared under similar (common garden) conditions (20 °C). The traits measured were: chill coma temperature ( CT min ), lethal temperature ( LT e 50 ), lethal time at low temperature ( LT i 50 ), chill coma recovery time ( CCRT ) and supercooling point ( SCP ). Measures of CT min , LT e 50 and LT i 50 proved to be the best predictors to describe the variation in realized latitudinal distributions ( R 2 = 0·699, R 2 = 0·741 and 0·550, respectively) and estimated environmental cold exposure ( R 2 = 0·633, R 2 = 0·641 and 0·511, respectively). Measures of CCRT also correlated significantly with estimated minimum temperature ( R 2 = 0·373), while the SCP did not. These results remained consistent after phylogenetically independent analysis or when applying nonlinear regression. Moreover, our findings were supported by a similar analysis based on existing data compiled from the Drosophila cold tolerance literature. Trait correlations were strong between LT e 50 , LT i 50 and CT min , respectively (0·83 > R 2 > 0·55). However, surprisingly, there was only a weak correlation between the entrance into coma ( CT min ) and the recovery from chill coma ( CCRT ) ( R 2 = 0·256). Considering the findings of the present study, data from previous studies and the logistical constraints of each measure of cold tolerance, we conclude that CT min and LT e 50 are superior measures when estimating the ecologically relevant cold tolerance of drosophilids. Of these two traits, CT min requires less equipment, time and animals and thereby presents a relatively fast, simple and dynamic measure of cold tolerance.
Год издания: 2014
Авторы: Jonas Lembcke Andersen, Tommaso Manenti, Jesper Givskov Sørensen, Heath A. MacMillan, Volker Loeschcke, Johannes Overgaard
Издательство: Wiley
Источник: Functional Ecology
Ключевые слова: Physiological and biochemical adaptations, Species Distribution and Climate Change, Neurobiology and Insect Physiology Research
Открытый доступ: bronze
Том: 29
Выпуск: 1
Страницы: 55–65