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RadiofrequencyThermal Ablation ofHepatocellularCarcinoma LiverNodules Can Activateand EnhanceTumor-Specific T-CellResponses Alessandro ZerbiniMassimo PilliAmalia Penna2006 год

Radiofrequency Thermal Ablation of Hepatocellular Carcinoma Liver Nodules Can Activate and Enhance Tumor-Specific T-Cell Responses
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Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: Abstract Radiofrequency thermal ablation (RFA) destroys tumoral tissue generating a local necrosis followed by marked inflammatory response with a dense T-cell infiltrate. In this study, we tested whether hepatocellular carcinoma thermal ablation can induce or enhance T-cell responses specific for hepatocellular carcinoma–associated antigens. Peripheral blood mononuclear cells derived from 20 patients with hepatocellular carcinoma were stimulated before and a month after RFA treatment with autologous hepatocellular carcinoma–derived protein lysates obtained before and immediately after RFA treatment. The effect of thermal ablation on memory T-cell responses to recall antigens [tetanus toxoid, protein purified derivative (PPD), Escherichia coli] was also assessed. T-cell reactivity was analyzed in an IFN-γ enzyme-linked immunospot assay and by intracellular IFN-γ staining. Treatment was followed by a significant increase of patients responsive either to tumor antigens derived from both the untreated hepatocellular carcinoma tissue (P < 0.05) and the necrotic tumor (P < 0.01) and by a higher frequency of circulating tumor-specific T cells. T-cell responses to recall antigens were also significantly augmented. Phenotypic analysis of circulating T and natural killer cells showed an increased expression of activation and cytotoxic surface markers. However, tumor-specific T-cell responses were not associated with protection from hepatocellular carcinoma relapse. Evidence of tumor immune escape was provided in one patient by the evidence that a new nodule of hepatocellular carcinoma recurrence was not recognized by T cells obtained at the time of RFA. In conclusion, RFA treatment generates the local conditions for activating the tumor-specific T-cell response. Although this effect is not sufficient for controlling hepatocellular carcinoma, it may represent the basis for the development of an adjuvant immunotherapy in patients undergoing RFA for primary and secondary liver tumors. (Cancer Res 2006; 66(2): 1139-46)
Год издания: 2006
Авторы: Alessandro Zerbini, Massimo Pilli, Amalia Penna, Guido Pelosi, Claudia Schianchi, A. Molinari, Simona Schivazappa, Carlo Zibera, Francesco Fagnoni, Carlo Ferrari, Gabriele Missale
Издательство: American Association for Cancer Research
Источник: Cancer Research
Ключевые слова: Immune Cell Function and Interaction, Immunotherapy and Immune Responses, Cancer Immunotherapy and Biomarkers
Другие ссылки: Cancer Research (PDF)
Cancer Research (HTML)
PubMed (HTML)