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N-Glycosylation of amouse IgG expressedin transgenic tobaccoplants Marion Cabanes‐MacheteauAnne‐Catherine Fitchette‐LainéCorinne Loutelier‐Bourhis1999 год

N-Glycosylation of a mouse IgG expressed in transgenic tobacco plants
статья из журнала

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: Since plants are emerging as an important system for the expression of recombinant glycoproteins, especially those intended for therapeutic purposes, it is important to scrutinize to what extent glycans harbored by mammalian glycoproteins produced in transgenic plants differ from their natural counterpart. We report here the first detailed analysis of the glycosylation of a functional mammalian glycoprotein expressed in a transgenic plant. The structures of the N-linked glycans attached to the heavy chains of the monoclonal antibody Guy's 13 produced in transgenic tobacco plants (plantibody Guy's 13) were identified and compared to those found in the corresponding IgG1 of murine origin. Both N-glycosylation sites located on the heavy chain of the plantibody Guy's 13 are N-glycosylated as in mouse. However, the number of Guy's 13 glycoforms is higher in the plant than in the mammalian expression system. Despite the high structural diversity of the plantibody N-glycans, glycosylation appears to be sufficient for the production of a soluble and biologically active IgG in the plant system. In addition to high-mannose-type N-glycans, 60% of the oligosaccharides N-linked to the plantibody have beta(1, 2)-xylose and alpha(1, 3)-fucose residues linked to the core Man3GlcNAc2. These plant-specific oligosaccharide structures are not a limitation to the use of plantibody Guy's 13 for topical immunotherapy. However, their immunogenicity may raise concerns for systemic applications of plantibodies in human.
Год издания: 1999
Авторы: Marion Cabanes‐Macheteau, Anne‐Catherine Fitchette‐Lainé, Corinne Loutelier‐Bourhis, C. Lange, Nicholas D. Vine, Jian-feng Ma, Patrice Lerouge, Loı̈c Faye
Издательство: Oxford University Press
Источник: Glycobiology
Ключевые слова: Transgenic Plants and Applications, Animal Genetics and Reproduction, Plant tissue culture and regeneration
Другие ссылки: Glycobiology (PDF)
Glycobiology (HTML)
PubMed (HTML)